Anti-RPA70 抗体 (ab87272)
Key features and details
- Rabbit polyclonal to RPA70
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RPA70 antibody
RPA70 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RPA70 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan
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免疫原
Synthetic peptide, corresponding to a region within amino acids 350 - 400 of Human RPA70 (NP_002936.1)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab87272の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/100 - 1/500. Antigen retrieval is not essential but may optimise staining. Antigen retrieval with tris-EDTA buffer pH 9.0 recommended. Likely to work with frozen sections.
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| 特記事項 |
|---|
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IHC-P
1/100 - 1/500. Antigen retrieval is not essential but may optimise staining. Antigen retrieval with tris-EDTA buffer pH 9.0 recommended. Likely to work with frozen sections. |
ターゲット情報
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機能
Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. -
配列類似性
Belongs to the replication factor A protein 1 family. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR.
Sumoylated on lysine residues Lys-449 and Lys-577, with Lys-449 being the major site. Sumoylation promotes recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombinaison. Desumoylated by SENP6. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 6117 Human
- Entrez Gene: 68275 Mouse
- Omim: 179835 Human
- SwissProt: P27694 Human
- SwissProt: Q8VEE4 Mouse
- Unigene: 461925 Human
- Unigene: 180734 Mouse
- Unigene: 472099 Mouse
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別名
- Dmrpa1 antibody
- Drosophila Replication Protein A antibody
- DRPA antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA70 antibody (ab87272)ab87272 at 1/250 dilution staining RPA70 in formalin-fixed, paraffin-embedded Human testicular seminoma tissue.
Detection: DAB staining. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA70 antibody (ab87272)ab87272 at 1/250 dilution staining RPA70 in formalin-fixed, paraffin-embedded Mouse colon carcinoma tissue.
Detection: DAB staining.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab87272 は 8 報の論文で使用されています。
- Takemoto K et al. Meiosis-Specific C19orf57/4930432K21Rik/BRME1 Modulates Localization of RAD51 and DMC1 to DSBs in Mouse Meiotic Recombination. Cell Rep 31:107686 (2020). WB ; Mouse . PubMed: 32460033
- Xu Y et al. A cell-based high-content screen identifies isocotoin as a small molecule inhibitor of the meiosis-specific MEIOB-SPATA22 complex†. Biol Reprod N/A:N/A (2020). PubMed: 32463099
- Qin Y et al. WDR62 is involved in spindle assembly by interacting with CEP170 in spermatogenesis. Development 146:N/A (2019). PubMed: 31533924
- Hua R et al. FBXO47 regulates telomere-inner nuclear envelope integration by stabilizing TRF2 during meiosis. Nucleic Acids Res 47:11755-11770 (2019). PubMed: 31724724
- Tian H et al. CXXC1 is not essential for normal DNA double-strand break formation and meiotic recombination in mouse. PLoS Genet 14:e1007657 (2018). PubMed: 30365547
- Xu Z et al. H2B ubiquitination regulates meiotic recombination by promoting chromatin relaxation. Nucleic Acids Res 44:9681-9697 (2016). PubMed: 27431324
- Luo M et al. MEIOB exhibits single-stranded DNA-binding and exonuclease activities and is essential for meiotic recombination. Nat Commun 4:2788 (2013). PubMed: 24240703
- Shin YH et al. Hormad1 mutation disrupts synaptonemal complex formation, recombination, and chromosome segregation in mammalian meiosis. PLoS Genet 6:e1001190 (2010). ICC/IF ; Mouse . PubMed: 21079677