Anti-RPA70 抗体 (ab12320)
Key features and details
- Rabbit polyclonal to RPA70
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-RPA70 antibody
RPA70 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RPA70 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan
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免疫原
Immunogen was a synthetic peptide, which represented a portion of human replication protein A1, 70kDa encoded within exon 12 (LocusLink ID 6117).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified using the peptide immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12320の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
1/5000 - 1/25000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
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| 特記事項 |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/5000 - 1/25000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa). |
ターゲット情報
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機能
Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. -
配列類似性
Belongs to the replication factor A protein 1 family. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR.
Sumoylated on lysine residues Lys-449 and Lys-577, with Lys-449 being the major site. Sumoylation promotes recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombinaison. Desumoylated by SENP6. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 6117 Human
- Entrez Gene: 68275 Mouse
- Omim: 179835 Human
- SwissProt: P27694 Human
- SwissProt: Q8VEE4 Mouse
- Unigene: 461925 Human
- Unigene: 180734 Mouse
- Unigene: 472099 Mouse
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別名
- Dmrpa1 antibody
- Drosophila Replication Protein A antibody
- DRPA antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA70 antibody (ab12320)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma (left) and mouse CT26 colon carcinoma (right) tissues labelling RPA70 with ab12320 at 1/1000 (1 µg/ml). Detection: DAB.
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Western blot - Anti-RPA70 antibody (ab12320)Detection of Human RPA70 by Western Blot. Samples: Whole cell lysate (10, 50 and 100 mcg/lane in lanes 1, 2 and 3, respectively) from HeLa cells. Antibody: ab12320 used at the indicated concentrations. Detection: Chemiluminescence with a 10 second exposure.
Detection of Human RPA70 by Western Blot.
Samples: Whole cell lysate (10, 50 and 100 mcg/lane in lanes 1, 2 and 3, respectively) from HeLa cells.
Antibody: ab12320 used at the indicated concentrations.
Detection: Chemiluminescence with a 10 second exposure.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab12320 は 7 報の論文で使用されています。
- Song J et al. Loss of RPA1 Impairs Peripheral T Cell Homeostasis and Exacerbates Inflammatory Damage through Triggering T Cell Necroptosis. Adv Sci (Weinh) 10:e2206344 (2023). PubMed: 36721037
- Sule A et al. Targeting IDH1/2 mutant cancers with combinations of ATR and PARP inhibitors. NAR Cancer 3:zcab018 (2021). PubMed: 34027408
- Velegzhaninov IO et al. Radioresistance, DNA Damage and DNA Repair in Cells With Moderate Overexpression of RPA1. Front Genet 11:855 (2020). PubMed: 32849834
- Nam B et al. TGFß1 Suppressed Matrix Mineralization of Osteoblasts Differentiation by Regulating SMURF1-C/EBPß-DKK1 Axis. Int J Mol Sci 21:N/A (2020). PubMed: 33371439
- Scarzello AJ et al. A Mutation in the SH2 domain of STAT2 prolongs tyrosine phosphorylation of STAT1 and promotes type I IFN-induced apoptosis. Mol Biol Cell 18:2455-62 (2007). WB ; Human . PubMed: 17442890
- McCabe N et al. Deficiency in the repair of DNA damage by homologous recombination and sensitivity to poly(ADP-ribose) polymerase inhibition. Cancer Res 66:8109-15 (2006). PubMed: 16912188
- Saeki H et al. Suppression of the DNA repair defects of BRCA2-deficient cells with heterologous protein fusions. Proc Natl Acad Sci U S A 103:8768-73 (2006). WB ; Human . PubMed: 16731627