Anti-RPA32/RPA2 抗体 (ab10359)
Key features and details
- Rabbit polyclonal to RPA32/RPA2
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RPA32/RPA2 antibody
RPA32/RPA2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RPA32/RPA2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rabbit, Horse, Pig, Chimpanzee, Rhesus monkey, Gorilla, Bat, Elephant
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免疫原
Synthetic peptide corresponding to Human RPA32/RPA2 aa 200 to the C-terminus (C terminal).
Database link: P15927 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10359の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/2500. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
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| IP |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
1/500 - 1/2500. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). |
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IP
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. -
翻訳後修飾
Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1. -
細胞内局在
Nucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage. - Information by UniProt
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参照データベース
- Entrez Gene: 456680 Chimpanzee
- Entrez Gene: 101144875 Gorilla
- Entrez Gene: 6118 Human
- Entrez Gene: 19891 Mouse
- Entrez Gene: 100347550 Rabbit
- Entrez Gene: 716612 Rhesus monkey
- Omim: 179836 Human
- SwissProt: P15927 Human
see all -
別名
- 60S acidic ribosomal protein P1 antibody
- AA409079 antibody
- AI325195 antibody
see all
画像
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Western blot - Anti-RPA32/RPA2 antibody (ab10359)All lanes : Anti-RPA32/RPA2 antibody (ab10359) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : Mouse TCMK-1 whole cell lysate
Lane 5 : Mouse NIH 3T3 whole cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 32 kDa
Exposure time: 10 seconds -
Immunoprecipitation - Anti-RPA32/RPA2 antibody (ab10359)HeLa whole cell lysate cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-).
Affinity purified rabbit anti-RPA32 antibody (3 µg) and rabbit anti-Phospho RPA32 antibody (1/100 dilution) were used.
Detection: Chemiluminescence
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody (ab10359)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling RPA32/RPA2 with ab10359 at 1/5000 (0.2µg/ml). Detection: DAB.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab10359 は 17 報の論文で使用されています。
- Benitez A et al. GEN1 promotes common fragile site expression. Cell Rep 42:112062 (2023). PubMed: 36729836
- Valero-Regalón FJ et al. Divergent patterns of meiotic double strand breaks and synapsis initiation dynamics suggest an evolutionary shift in the meiosis program between American and Australian marsupials. Front Cell Dev Biol 11:1147610 (2023). PubMed: 37181752
- Davies B et al. Characterization of meiotic recombination intermediates through gene knockouts in founder hybrid mice. Genome Res 33:2018-2027 (2023). PubMed: 37977820
- Fowler FC et al. DNA-PK promotes DNA end resection at DNA double strand breaks in G0 cells. Elife 11:N/A (2022). PubMed: 35575473
- Llerena Cari E et al. Integrated stress response control of granulosa cell translation and proliferation during normal ovarian follicle development. Mol Hum Reprod 27:N/A (2021). PubMed: 34314477