AB97594

Anti-RPA32/RPA2 抗体

Name: Anti-RPA32/RPA2 抗体 (ab97594) | Abcam
Brand: Abcam Limited
SKU: AB97594
Price: 78000 JPY
Availability: InStock

Anti-RPA32/RPA2 antibody

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(3 Publications)

Rabbit Polyclonal RPA32/RPA2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RPA2 aa 1 to C-terminus.

別名を表示する

REPA2, RPA32, RPA34, RPA2, Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, Replication protein A 34 kDa subunit, RF-A protein 2, RP-A p34

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody (AB97594)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody (AB97594)

ab97594, at a 1/100 dilution, staining human RPA32/RPA2 in A549 xenograft, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody (AB97594)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody (AB97594)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling RPA32 protein at nucleus using ab97594 at a 1/500 dilution (Green).
alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/1000 dilution (Red).

Supplier Data

Western blot - Anti-RPA32/RPA2 antibody (AB97594)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-RPA32/RPA2 antibody (ab97594) at 1/1000 dilution

All lanes:

293T Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 29 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, IHC-P, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human RPA2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P15927

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed : 17596542).

See full target information RPA2

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 84:3044-3060.e11 PubMed39142279

2024

HLTF resolves G4s and promotes G4-induced replication fork slowing to maintain genome stability.

Applications

Unspecified application

Species

Unspecified reactive species

Gongshi Bai,Theresa Endres,Ulrike Kühbacher,Valentina Mengoli,Briana H Greer,Emma M Peacock,Matthew D Newton,Tyler Stanage,Maria Rosaria Dello Stritto,Roxana Lungu,Magdalena P Crossley,Ataya Sathirachinda,David Cortez,Simon J Boulton,Petr Cejka,Brandt F Eichman,Karlene A Cimprich

Cells 8: PubMed31035352

2019

Telomere Function and the G-Quadruplex Formation are Regulated by hnRNP U.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroto Izumi,Keiko Funa

Nature cell biology 20:92-103 PubMed29255170

2017

Unresolved recombination intermediates lead to ultra-fine anaphase bridges, chromosome breaks and aberrations.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Wai Chan,Kasper Fugger,Stephen C West

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPA32/RPA2 抗体

Anti-RPA32/RPA2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody (AB97594)

Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody (AB97594)

Western blot - Anti-RPA32/RPA2 antibody (AB97594)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (3)

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Abcam product promise