Anti-Rotavirus 抗体 [A2] (ab181695)
Key features and details
- Mouse monoclonal [A2] to Rotavirus
- Suitable for: WB, ICC/IF
- Reacts with: Rotavirus
- Isotype: IgG2b
製品の概要
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製品名
Anti-Rotavirus antibody [A2]
Rotavirus 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [A2] to Rotavirus -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Rotavirus -
免疫原
Recombinant fragment corresponding to Rotavirus. Inner capside protein VP6.
Database link: P04509 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Constituents: 0.75% Glycine, 2% Sucrose, 1.21% Tris -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
A2 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181695の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/200 - 1/1000. Predicted molecular weight: 45 kDa.
|
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| ICC/IF | (1) |
1/100 - 1/500.
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| 特記事項 |
|---|
|
WB
1/200 - 1/1000. Predicted molecular weight: 45 kDa. |
|
ICC/IF
1/100 - 1/500. |
ターゲット情報
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関連性
Rotaviruses, members of the family Reoviridae, are a major cause of diarrhoea in young mammals. Rotavirus infections also result in economic losses in agriculture due to diarrhoea in calf, pig, sheep, and poultry rearing. Diarrhoea (or scours) due to the rotavirus Nebraska Calf Diarrhea Virus can affect calves up to 30 days of age or older. Diarrhoea begins 2 to 3 days after exposure. Diagnosis is by history, lesions (ulcers on the tongue, lips, and mouth) and diagnostic laboratory tests. Mortality rates may be as high as 50 percent, depending on the secondary bacteria present. Human rotaviruses, the major aetiological agents of severe infantile diarrhoea worldwide, display surprisingly diverse and complex serotypic specificities. Rotaviruses are 70 nm, non enveloped viruses comprised of a triple layered protein capsid; Outer capsid proteins are VP4 and VP7, Inner capsid -VP6 and Core -VP2. The immunity acquired from exposure to rotavirus appears to be type specific following initial infection; therefore, multiple serotypes of rotavirus mean multiple opportunities for infection. The combination of animal reservoirs for the virus and rotavirus gene reassortment provides the potential for dramatic genetic shifts (similar to influenza virus) which could give rise to altered host ranges and viral virulence. -
別名
- Major inner capsid protein VP6 antibody
- NSP4 antibody
- VP6 antibody
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab181695 は 9 報の論文で使用されています。
- Zhang R et al. Metformin Hydrochloride Significantly Inhibits Rotavirus Infection in Caco2 Cell Line, Intestinal Organoids, and Mice. Pharmaceuticals (Basel) 16:N/A (2023). PubMed: 37765086
- Bruno C et al. Protective effects of the postbiotic deriving from cow's milk fermentation with L. paracasei CBA L74 against Rotavirus infection in human enterocytes. Sci Rep 12:6268 (2022). PubMed: 35428750
- Mukhopadhyay U et al. Rotavirus activates MLKL-mediated host cellular necroptosis concomitantly with apoptosis to facilitate dissemination of viral progeny. Mol Microbiol 117:818-836 (2022). PubMed: 34954851
- Diebold O et al. Using Species a Rotavirus Reverse Genetics to Engineer Chimeric Viruses Expressing SARS-CoV-2 Spike Epitopes. J Virol 96:e0048822 (2022). PubMed: 35758692
- Yamada K et al. Screening for Components/Compounds with Anti-Rotavirus Activity: Detection of Interaction Between Viral Spike Proteins and Glycans. Methods Mol Biol 2132:585-595 (2020). PubMed: 32306358
- Li Z et al. A milk-based self-assemble rotavirus VP6-ferritin nanoparticle vaccine elicited protection against the viral infection. J Nanobiotechnology 17:13 (2019). PubMed: 30670042
- Hakim MS et al. Basal interferon signaling and therapeutic use of interferons in controlling rotavirus infection in human intestinal cells and organoids. Sci Rep 8:8341 (2018). PubMed: 29844362
- Hakim MS et al. TNF-a exerts potent anti-rotavirus effects via the activation of classical NF-?B pathway. Virus Res 253:28-37 (2018). ICC/IF . PubMed: 29859235
- Gao J et al. MYH9 is an Essential Factor for Porcine Reproductive and Respiratory Syndrome Virus Infection. Sci Rep 6:25120 (2016). PubMed: 27112594