Anti-ROR2 抗体 (ab245456)
Key features and details
- Rabbit polyclonal to ROR2
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ROR2 antibody
ROR2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ROR2 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human ROR2 aa 893-943. The exact sequence is proprietary. NP_004551.2
Database link: Q01974 -
ポジティブ･コントロール
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab245456 was affinity purified using an epitope specific to ROR2 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245456の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 105 kDa.
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| 特記事項 |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 105 kDa. |
ターゲット情報
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機能
Tyrosine-protein kinase receptor which may be involved in the early formation of the chondrocytes. It seems to be required for cartilage and growth plate development. Phosphorylates YWHAB, leading to induction of osteogenesis and bone formation. -
関連疾患
Brachydactyly B1
Robinow syndrome autosomal recessive -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily.
Contains 1 FZ (frizzled) domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 kringle domain.
Contains 1 protein kinase domain. -
発生段階
Expressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4920 Human
- Omim: 602337 Human
- SwissProt: Q01974 Human
- Unigene: 644776 Human
- Unigene: 98255 Human
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別名
- BDB antibody
- BDB1 antibody
- Brachydactyly type B antibody
see all
画像
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Western blot - Anti-ROR2 antibody (ab245456)All lanes : Anti-ROR2 antibody (ab245456) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 105 kDa
Exposure time: 10 seconds -
Immunoprecipitation - Anti-ROR2 antibody (ab245456)ROR2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245456 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245456 at 1 µg/ml.
Lane 1: ab245456 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with exposure time of 10 seconds.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab245456 は 2 報の論文で使用されています。
- Yang H et al. Circ_0044520 regulates the progression of laryngeal squamous cell carcinoma via the miR-338-3p/ROR2 axis. Histol Histopathol 37:513-526 (2022). PubMed: 35037694
- Tang Y et al. Long Noncoding RNA TUG1 Promotes the Function in ox-LDL-Treated HA-VSMCs via miR-141-3p/ROR2 Axis. Cardiovasc Ther 2020:6758934 (2020). PubMed: 32565910