Anti-ROR beta/RORB 抗体 [EPR15552] - N-terminal (ab187657)
![Immunocytochemistry/ Immunofluorescence - Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657)](https://www.abcam.co.jp/ps/products/187/ab187657/Images/ab187657-530177-antiRORbetaRORB-antibody-epr15552-immunocytochemistry-immunofluorescence-human-hepg2.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15552] to ROR beta/RORB - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ROR beta/RORB antibody [EPR15552] - N-terminal
ROR beta/RORB 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR15552] to ROR beta/RORB - N-terminal -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
非交差種: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HepG2 whole cell lysate (ab7900) and human cerebellum tissue lysate;
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR15552 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187657の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/2000. Predicted molecular weight: 53 kDa.
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| ICC/IF |
1/1000.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/2000. Predicted molecular weight: 53 kDa. |
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ICC/IF
1/1000. |
ターゲット情報
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機能
Orphan nuclear receptor required for normal postnatal development of rod and cone photoreceptor cells. Regulates transcription of OPN1SW in cone photoreceptor cells by binding the sequence 5'-AGGTCA-3' in the OPN1SW promoter. -
配列類似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 6096 Human
- Omim: 601972 Human
- SwissProt: Q92753 Human
- Unigene: 494178 Human
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別名
- bA133M9.1 antibody
- NR1F2 antibody
- Nuclear receptor ROR-beta antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657)
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) labelling ROR beta/RORB with ab187657 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488 (ab150077) secondary antibody at 1/1000 dilution (green).
Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution was used as a counterstain (red).
The nuclear counterstain is DAPI (blue).
Confocal image showing nuclear staining in HepG2 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Western blot - Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657)](https://www.abcam.co.jp/ps/products/187/ab187657/Images/ab187657-220694-anti-ror-beta-antibody-epr15552-n-terminal-western-blot.jpg)
Western blot - Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657)Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657) at 1/1000 dilution + HepG2 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 53 kDa -
![Western blot - Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657)](https://www.abcam.co.jp/ps/products/187/ab187657/Images/ab187657-220695-anti-ror-beta-antibody-epr15552-n-terminal-western-blot.jpg)
Western blot - Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657)Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657) at 1/1000 dilution + Human cerebellum tissue lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 53 kDa -
![Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657)](https://www.abcam.co.jp/ps/products/187/ab187657/Images/ab187657-5-benefits-of-recombinant-antibodies.png)
Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab187657 は 1 報の論文で使用されています。
- Zheng X et al. Preclinical long-term safety of intraspinal transplantation of human dorsal spinal GABA neural progenitor cells. iScience 26:108306 (2023). PubMed: 38026209