Anti-ROR alpha/RORA 抗体 (ab70061)
Key features and details
- Rabbit polyclonal to ROR alpha/RORA
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
製品の概要
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製品名
Anti-ROR alpha/RORA antibody
ROR alpha/RORA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ROR alpha/RORA -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human, African green monkey -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human breast carcinoma tissue, extracts from HeLa and COS-7 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70061の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
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| IHC-P |
1/50 - 1/100.
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa). |
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IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
Orphan nuclear receptor. Binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence. This isomer binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPARgamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM AND ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1. Possible receptor for cholesterol or one of its derivatives. -
組織特異性
Widely expressed in a number of tissues. -
配列類似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
翻訳後修飾
Phosphorylation by PKC in neurons inhibits transcriptional activity. Phosphorylated on Thr-216 by ERK2 in vitro.
Sumoylated by SENP1 and SENP2. Sumoylation, promoted by PIAS2, PIAS3, PIASy but not PIAS1, enhances the transcriptional activity. Desumoylated by SENP1.
Ubiquitinated. Ubiquitination is required for efficient transcriptional activity and is prevented by Hairless. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 6095 Human
- Omim: 600825 Human
- SwissProt: P35398 Human
- Unigene: 560343 Human
- Unigene: 655155 Human
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別名
- fhl2a antibody
- NR1F1 antibody
- Nuclear receptor ROR alpha antibody
see all
画像
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Western blot - Anti-ROR alpha/RORA antibody (ab70061)All lanes : Anti-ROR alpha/RORA antibody (ab70061) at 1/500 dilution
Lane 1 : extract from HeLa cells
Lane 2 : extract from COS-7 cells
Lane 3 : extract from COS-7 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 63 kDa
Observed band size: 63 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR alpha/RORA antibody (ab70061)Human breast carcinoma tissue with ab70061 at 1/50 dilution, with and without the immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab70061 は 3 報の論文で使用されています。
- Zhang Z et al. A novel immune-related prognostic signature based on Chemoradiotherapy sensitivity predicts long-term survival in patients with esophageal squamous cell carcinoma. PeerJ 11:e15839 (2023). PubMed: 37609436
- Li D et al. RORA alleviates LPS-induced apoptosis of renal epithelial cells by promoting PGC-1α transcription. Clin Exp Nephrol 26:512-521 (2022). PubMed: 35195816
- Kang X et al. Sevoflurane blocks glioma malignant development by upregulating circRELN through circRELN-mediated miR-1290/RORA axis. BMC Anesthesiol 21:213 (2021). PubMed: 34479497