AB124671

Anti-RON (phospho Y1353) 抗体 [EPR5598]

Anti-RON (phospho Y1353) antibody [EPR5598]

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal RON phospho Y1353 antibody. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 3 publications.

別名を表示する

CD136, PTK8, RON, MST1R, Macrophage-stimulating protein receptor, MSP receptor, CDw136, Protein-tyrosine kinase 8, p185-Ron

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-RON (phospho Y1353) antibody [EPR5598] (AB124671)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RON (phospho Y1353) antibody [EPR5598] (AB124671)

ab124671, at 1/500 dilution, staining RON in A431 (Human epidermoid carcinoma cell line) cells by Immunofluorescence.

Unknown

Western blot - Anti-RON (phospho Y1353) antibody [EPR5598] (AB124671)

All lanes:

Western blot - Anti-RON (phospho Y1353) antibody [EPR5598] (ab124671) at 1/50000 dilution

Lane 1:

A431 cell lysates, untreated

Lane 2:

A431 cell lysates, treated with pervanadate

Predicted band size: 152 kDa

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OI-RD Scanning - Anti-RON (phospho Y1353) antibody [EPR5598] (AB124671)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-RON (phospho Y1353) antibody [EPR5598] (AB124671)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR5598

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000 - 1/200000", "WB-species-notes": "" } } }

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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to MST1 ligand. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces autophosphorylation of RON on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1 or the adapter GAB1. Recruitment of these downstream effectors by RON leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. RON signaling activates the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. Also plays a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Alternatively, RON can also promote signals such as cell migration and proliferation in response to growth factors other than MST1 ligand.

See full target information MST1R phospho Y1353

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 13:351 PubMed35428350

2022

EGFR/MET promotes hepatocellular carcinoma metastasis by stabilizing tumor cells and resisting to RTKs inhibitors in circulating tumor microemboli.

Applications

Unspecified application

Species

Unspecified reactive species

Shouyang Song,Zhen Yu,Yajing You,Chenxi Liu,Xiaoyu Xie,Huanran Lv,Feng Xiao,Qiang Zhu,Chengyong Qin

Experimental and therapeutic medicine 22:798 PubMed34093754

2021

The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR.

Applications

Unspecified application

Species

Unspecified reactive species

Donghui Zhou,Xiaohua Zhu,Xuan Wu,Jingjing Zheng,Laizhen Tou,Yong Zhou

PloS one 13:e0191983 PubMed29408900

2018

Phosphoproteomic analysis reveals Smad protein family activation following Rift Valley fever virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Cynthia de la Fuente,Chelsea Pinkham,Deemah Dabbagh,Brett Beitzel,Aura Garrison,Gustavo Palacios,Kimberley Alex Hodge,Emanuel F Petricoin,Connie Schmaljohn,Catherine E Campbell,Aarthi Narayanan,Kylene Kehn-Hall

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RON (phospho Y1353) 抗体 [EPR5598]

Anti-RON (phospho Y1353) antibody [EPR5598]

Immunocytochemistry/ Immunofluorescence - Anti-RON (phospho Y1353) antibody [EPR5598] (AB124671)

Western blot - Anti-RON (phospho Y1353) antibody [EPR5598] (AB124671)

OI-RD Scanning - Anti-RON (phospho Y1353) antibody [EPR5598] (AB124671)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (3)

Abcam product promise