Anti-RON 抗体 [EPR20954]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20954] (AB227979)

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling RON with ab227979 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Membranous staining on human bladder cancer cells is observed (PMID : 15870710). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20954] (AB227979)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling RON with ab227979 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Cytoplasmic and membranous staining in germinal centers of human tonsil is observed (PMID : 9890710). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20954] (AB227979)

Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling RON with ab227979 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Cytoplasmic and membranous staining in human gastric epithelium is observed (PMID : 9890710). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-RON antibody [EPR20954] (AB227979)

Exposure times : Lane 1 : 10 seconds; Lane 2 : 58 seconds; Lanes 3,5 : 3 minutes; Lane 4 : 2 minutes.

Blocking/Dilution buffer : 5% NFDM/TBST.

The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

The 185kDa RON precursor protein is cleaved into a 40kDa α and 150kDa β chain, which are linked by disulphide bonds in the 180kDa mature form (PMID : 9890710, PMID : 23792360, PMID : 17889431). There are several isoforms of RON, including a 110kDa truncation (23792360). ab227979 is specific for the β chain.

Lanes 1 - 4:

Western blot - Anti-RON antibody [EPR20954] (ab227979) at 1/5000 dilution

Lane 5:

Western blot - Anti-RON antibody [EPR20954] (ab227979) at 1/1000 dilution

Lane 1:

BxPC-3 (human pancreas adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 2:

SK-BR-3 (human mammary gland adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 3:

SW480 (human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 4:

HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 20 µg

Lane 5:

Human stomach tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 152 kDa

Observed band size: 100 kDa,152 kDa

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