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AB228855

Anti-RON 抗体 [EPR20953] - BSA and Azide free

Anti-RON antibody [EPR20953] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal RON phospho Y1353 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

別名を表示する

CD136, PTK8, RON, MST1R, Macrophage-stimulating protein receptor, MSP receptor, CDw136, Protein-tyrosine kinase 8, p185-Ron

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20953] - BSA and Azide free (AB228855)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20953] - BSA and Azide free (AB228855)

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling RON with ab227980 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Membranous staining on human bladder cancer cells is observed (PMID : 15870710). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227980).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20953] - BSA and Azide free (AB228855)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20953] - BSA and Azide free (AB228855)

Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling RON with ab227980 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Cytoplasmic and membranous staining in human gastric epithelium is observed (PMID : 9890710). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227980).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20953] - BSA and Azide free (AB228855)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20953] - BSA and Azide free (AB228855)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling RON with ab227980 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Cytoplasmic and membranous staining in germinal centers of human tonsil is observed (PMID : 9890710). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227980).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR20953

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

ab227980 detects a distinct immunogen compared to ab227979.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval using Universal HIER antigen retrieval reagent (10X) (<a href='/products/ihc-kits/universal-hier-antigen-retrieval-reagent-10x-ab208572'>ab208572</a>).</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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製品の詳細

ab228855 is the carrier-free version of ab227980.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to MST1 ligand. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces autophosphorylation of RON on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1 or the adapter GAB1. Recruitment of these downstream effectors by RON leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. RON signaling activates the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. Also plays a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Alternatively, RON can also promote signals such as cell migration and proliferation in response to growth factors other than MST1 ligand.
See full target information MST1R phospho Y1353
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Aging 14:2004-2013 PubMed35196650

2022

MKRN2 inhibits the proliferation of gastric cancer by downregulating PKM2.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Liu,Shuyao Xiang,Xingchen Guo,Jinghuan Zhou,Lixin Liao,Jiaxin Kou,Jun Zhang
View all publications
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代替製品

Primary Antibodies

AB199532

Anti-RON antibody [EPR16789]

RabMAb|Recombinant

Western blot - Anti-RON antibody [EPR16789] (AB199532)

  • 0
  • 0
Primary Antibodies

AB124671

Anti-RON (phospho Y1353) antibody [EPR5598]

RabMAb|Recombinant|Trial Size|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-RON (phospho Y1353) antibody [EPR5598] (AB124671)

  • 0
  • 0
Primary Antibodies

AB232028

Anti-RON (phospho Y1353) antibody [EPR5598] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-RON (phospho Y1353) antibody [EPR5598] - BSA and Azide free (AB232028)

  • 0
  • 0
Primary Antibodies

AB227979

Anti-RON antibody [EPR20954]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20954] (AB227979)

  • 0
  • 0
Primary Antibodies

AB228853

Anti-RON antibody [EPR20954] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20954] - BSA and Azide free (AB228853)

  • 0
  • 0
Primary Antibodies

AB52927

Anti-RON antibody [EP1132Y]

RabMAb|Recombinant|Trial Size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EP1132Y] (AB52927)

  • 3
  • 1

組合せ製品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB180953

Anti-PAR2 antibody [EPR13675]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR2 antibody [EPR13675] (AB180953)

  • 4
  • 1
Primary Antibodies

AB82176

Anti-TMPRSS4 antibody

Western blot - Anti-TMPRSS4 antibody (AB82176)

  • 0
  • 0
Primary Antibodies

AB133757

Anti-NPY5R antibody [EPR8382]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-NPY5R antibody [EPR8382] (AB133757)

  • 5
  • 2

Abcam product promise

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