AB199532

Anti-RON 抗体 [EPR16789]

Anti-RON antibody [EPR16789]

RabMAb
Recombinant
詳細を見る

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(1 Publication)

Rabbit Recombinant Monoclonal RON antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

別名を表示する

CD136, PTK8, RON, MST1R, Macrophage-stimulating protein receptor, MSP receptor, CDw136, Protein-tyrosine kinase 8, p185-Ron

2 Images

Supplier Data

Western blot - Anti-RON antibody [EPR16789] (AB199532)

Blocking/Dilution buffer : 5% NFDM/TBST.

Both MCF7 and MDA MB 435S are RON negative cell lines.

All lanes:

Western blot - Anti-RON antibody [EPR16789] (ab199532) at 1/1000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Lane 2:

MDA-MB-435S (Human ductal carcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 152 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-RON antibody [EPR16789] (AB199532)

Blocking/Dilution buffer : 5% NFDM/TBST.

The observed MW is consistent with what has been described in the literature PMID : 17616662.

All lanes:

Western blot - Anti-RON antibody [EPR16789] (ab199532) at 1/4000 dilution

Lane 1:

HT-29 (Human colorectal adenocarcinoma cells) whole cell lysate at 10 µg

Lane 2:

BxPC-3 (Human pancreas adenocarcinoma cells) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 152 kDa

Observed band size: 185 kDa

false

Exposure time: 30s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR16789

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/4000", "WB-species-notes": "<p></p>" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to MST1 ligand. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces autophosphorylation of RON on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1 or the adapter GAB1. Recruitment of these downstream effectors by RON leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. RON signaling activates the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. Also plays a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Alternatively, RON can also promote signals such as cell migration and proliferation in response to growth factors other than MST1 ligand.

See full target information MST1R

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 9:eade5041 PubMed37043568

2023

Milk-derived extracellular vesicles protect intestinal barrier integrity in the gut-liver axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lingjun Tong,Sitong Zhang,Qiqi Liu,Chenyuan Huang,Haining Hao,Michelle Siying Tan,Xiaodong Yu,Charles Kang Liang Lou,Rong Huang,Zhe Zhang,Tongjie Liu,Pimin Gong,Cheng Han Ng,Mark Muthiah,Giorgia Pastorin,Matthias Gerhard Wacker,Xiaoyuan Chen,Gert Storm,Cheun Neng Lee,Lanwei Zhang,Huaxi Yi,Jiong-Wei Wang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RON 抗体 [EPR16789]

Anti-RON antibody [EPR16789]

Western blot - Anti-RON antibody [EPR16789] (AB199532)

Western blot - Anti-RON antibody [EPR16789] (AB199532)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (1)

Abcam product promise