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リコンビナントRabMAb

Anti-Robo4 抗体 [EPR26282-3] (BSA and Azide free) (ab300047)

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Western blot - Anti-Robo4 antibody [EPR26282-3] (BSA and Azide free) (AB300047)
  • Western blot - Anti-Robo4 antibody [EPR26282-3] (BSA and Azide free) (AB300047)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo4 antibody [EPR26282-3] (BSA and Azide free) (AB300047)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo4 antibody [EPR26282-3] (BSA and Azide free) (AB300047)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo4 antibody [EPR26282-3] (BSA and Azide free) (AB300047)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo4 antibody [EPR26282-3] (BSA and Azide free) (AB300047)
  • Anti-Robo4 antibody [EPR26282-3] (BSA and Azide free) (AB300047)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26282-3] to Robo4
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Robo4 antibody [EPR26282-3] (BSA and Azide free)
    Robo4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26282-3] to Robo4

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WBmore details
    適用なし: Flow Cyt (Intra),ICC/IF,IHC-Fr or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HUVEC (human umbilical vein endothelial cell); Human heart tissue lysate; Mouse & Rat spleen tissue lysates; Mouse & Rat kidney tissue lysates. IHC-P: Human & Mouse colon; Mouse pancreatic cancer; Rat cerebrum

  • 特記事項

    ab300047 is a carrier free version of ab300046.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

関連製品

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300047の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 107 kDa.
特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 107 kDa.
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF,IHC-Fr or IP.

ターゲット情報

画像

  • Western blot - Anti-Robo4 antibody [EPR26282-3] (BSA and Azide free) (AB300047)
    Western blot - Anti-Robo4 antibody [EPR26282-3] (BSA and Azide free) (AB300047)
    All lanes : Anti-Robo4 antibody [EPR26282-3] (ab300046) at 1/1000 dilution

    Lane 1 : HUVEC (human umbilical vein endothelial cell) whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 107 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    This data was developed using ab300046, the same antibody clone in a different buffer formulation. 

    Blocking / Diluting buffer and concentration:

    5% NFDM/TBST

    Negative controls: HeLa, HCT 116(PMID: 11944987, PMID: 11076864 ).

  • Western blot - Anti-Robo4 antibody [EPR26282-3] (BSA and Azide free) (AB300047)
    Western blot - Anti-Robo4 antibody [EPR26282-3] (BSA and Azide free) (AB300047)
    All lanes : Anti-Robo4 antibody [EPR26282-3] (ab300046) at 1/1000 dilution

    Lane 1 : Human heart tissue lysate
    Lane 2 : Mouse spleen tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Rat spleen tissue lysate
    Lane 5 : Rat kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051);Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG. Secondary ab dilution 1:2000;1:20,000

    Predicted band size: 107 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    This data was developed using ab300046, the same antibody clone in a different buffer formulation. 

    Blocking / Diluting buffer and concentration:

    5% NFDM/TBST

    The molecular weight observed is consistent with what have been described in literature (PMID: 27882935).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo4 antibody [EPR26282-3] (BSA and Azide free) (AB300047)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo4 antibody [EPR26282-3] (BSA and Azide free) (AB300047)

    This data was developed using ab300046, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Robo4 with abab300046 at 1/100 (3.77 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Positive staining on endothelial cells in human colon. The section was incubated with ab300046 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo4 antibody [EPR26282-3] (BSA and Azide free) (AB300047)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo4 antibody [EPR26282-3] (BSA and Azide free) (AB300047)

    This data was developed using ab300046, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Robo4 with abab300046 at 1/100 (3.77 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on endothelial cells in mouse colon. The section was incubated with ab300046 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo4 antibody [EPR26282-3] (BSA and Azide free) (AB300047)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo4 antibody [EPR26282-3] (BSA and Azide free) (AB300047)

    This data was developed using ab300046, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse pancreatic can tissue labeling Robo4 with abab300046 at 1/100 (3.77 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on endothelial cells in mouse pancreatic cancer. The section was incubated with ab300046 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo4 antibody [EPR26282-3] (BSA and Azide free) (AB300047)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo4 antibody [EPR26282-3] (BSA and Azide free) (AB300047)

    This data was developed using ab300046, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Robo4 with abab300046 at 1/100 (3.77 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Positive staining on endothelial cells in rat cerebrum. The section was incubated with ab300046 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

  • Anti-Robo4 antibody [EPR26282-3] (BSA and Azide free) (AB300047)
    Anti-Robo4 antibody [EPR26282-3] (BSA and Azide free) (AB300047)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab300047 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab300047 は論文での使用が確認できていません。

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