Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-Robo4 抗体 [EPR26282-3] (ab300046)

Submit a review Submit a question
Western blot - Anti-Robo4 antibody [EPR26282-3] (AB300046)
  • Western blot - Anti-Robo4 antibody [EPR26282-3] (AB300046)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo4 antibody [EPR26282-3] (AB300046)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo4 antibody [EPR26282-3] (AB300046)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo4 antibody [EPR26282-3] (AB300046)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo4 antibody [EPR26282-3] (AB300046)
  • Anti-Robo4 antibody [EPR26282-3] (AB300046)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26282-3] to Robo4
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Robo4 antibody [EPR26282-3]
    Robo4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26282-3] to Robo4

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WBmore details
    適用なし: Flow Cyt (Intra),ICC/IF,IHC-Fr or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HUVEC (human umbilical vein endothelial cell); Human heart tissue lysate; Mouse & Rat spleen tissue lysates; Mouse & Rat kidney tissue lysates. IHC-P: Human & Mouse colon; Mouse pancreatic cancer; Rat cerebrum

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300046の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Predicted molecular weight: 107 kDa.
特記事項
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Predicted molecular weight: 107 kDa.
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF,IHC-Fr or IP.

ターゲット情報

画像

  • Western blot - Anti-Robo4 antibody [EPR26282-3] (AB300046)
    Western blot - Anti-Robo4 antibody [EPR26282-3] (AB300046)
    All lanes : Anti-Robo4 antibody [EPR26282-3] (ab300046) at 1/1000 dilution

    Lane 1 : HUVEC (human umbilical vein endothelial cell) whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 107 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking / Diluting buffer and concentration:

    5% NFDM/TBST

  • Western blot - Anti-Robo4 antibody [EPR26282-3] (AB300046)
    Western blot - Anti-Robo4 antibody [EPR26282-3] (AB300046)
    All lanes : Anti-Robo4 antibody [EPR26282-3] (ab300046) at 1/1000 dilution

    Lane 1 : Human heart tissue lysate
    Lane 2 : Mouse spleen tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Rat spleen tissue lysate
    Lane 5 : Rat kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051);Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG. Secondary ab dilution 1:2000;1:20,000

    Predicted band size: 107 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking / Diluting buffer and concentration:5% NFDM/TBST

    The molecular weight observed is consistent with what have been described in literature (PMID: 27882935).


    Negative controls: HeLa, HCT 116(PMID: 11944987, PMID: 11076864 ).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo4 antibody [EPR26282-3] (AB300046)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo4 antibody [EPR26282-3] (AB300046)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Robo4 with abab300046 at 1/100 (3.77 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on endothelial cells in human colon. The section was incubated with ab300046 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo4 antibody [EPR26282-3] (AB300046)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo4 antibody [EPR26282-3] (AB300046)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Robo4 with abab300046 at 1/100 (3.77 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on endothelial cells in mouse colon. The section was incubated with ab300046 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo4 antibody [EPR26282-3] (AB300046)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo4 antibody [EPR26282-3] (AB300046)

    Immunohistochemical analysis of paraffin-embedded Mouse pancreatic can tissue labeling Robo4 with abab300046 at 1/100 (3.77 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on endothelial cells in mouse pancreatic cancer. The section was incubated with ab300046 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo4 antibody [EPR26282-3] (AB300046)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo4 antibody [EPR26282-3] (AB300046)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Robo4 with abab300046 at 1/100 (3.77 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Positive staining on endothelial cells in rat cerebrum. The section was incubated with ab300046 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

  • Anti-Robo4 antibody [EPR26282-3] (AB300046)
    Anti-Robo4 antibody [EPR26282-3] (AB300046)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab300046 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab300046 は論文での使用が確認できていません。

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

There are currently no Customer reviews or Questions for ab300046.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com