Anti-Robo1 抗体 (ab7279)
Key features and details
- Rabbit polyclonal to Robo1
- Suitable for: ELISA, IHC-P, WB, IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Robo1 antibody
Robo1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Robo1 -
由来種
Rabbit -
特異性
Some batches may cross-react with Robo2 and Robo3. Please see abreview for more details.
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アプリケーション
適用あり: ELISA, IHC-P, WB, IHC-Frmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide:
C-VLGGYERGEDNNE
conjugated to KLH, corresponding to amino acids 1632-1644 of Human Robo1. -
ポジティブ･コントロール
- WB: HEK-293, HeLa, MCF-7, A-549, and A-172 lysates. IHC-P: Human brain tissue. IHC-Fr: Mouse lung tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This affinity purified antibody is directed against human ROBO-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7279の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
1/30000 - 1/60000.
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| IHC-P | (3) |
Use a concentration of 2 - 10 µg/ml.
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| WB | (4) |
1/500 - 1/3000. Detects a band of approximately 181 kDa.
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| IHC-Fr | (4) |
Use a concentration of 2 - 10 µg/ml.
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| 特記事項 |
|---|
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ELISA
1/30000 - 1/60000. |
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IHC-P
Use a concentration of 2 - 10 µg/ml. |
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WB
1/500 - 1/3000. Detects a band of approximately 181 kDa. |
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IHC-Fr
Use a concentration of 2 - 10 µg/ml. |
ターゲット情報
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機能
Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development. -
組織特異性
Widely expressed, with exception of kidney. -
配列類似性
Belongs to the immunoglobulin superfamily. ROBO family.
Contains 3 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
翻訳後修飾
Ubiquitinated. May be deubiquitinated by USP33. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 6091 Human
- Entrez Gene: 19876 Mouse
- Omim: 602430 Human
- SwissProt: Q9Y6N7 Human
- SwissProt: O89026 Mouse
- Unigene: 13640 Human
- Unigene: 310772 Mouse
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別名
- Deleted in U twenty twenty antibody
- DUTT 1 antibody
- DUTT1 antibody
see all
画像
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Western blot - Anti-Robo1 antibody (ab7279)Anti-Robo1 antibody (ab7279) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo1 antibody (ab7279)This image shows staining of human brain tissue.
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Immunohistochemistry (Frozen sections) - Anti-Robo1 antibody (ab7279)Immunohistochemistry analysis of mouse lung tissue sections (adult, frozen 100 µm wholemount sections) labelling Robo1 with ab7279 at 1/50 dilution. The tissue was paraformaldehyde fixed and permeabilized with triton x-100 before incubation with the antibody for 16 hours at 4°C.
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Western blot - Anti-Robo1 antibody (ab7279)This image is courtesy of an anonymous Abreview.Anti-Robo1 antibody (ab7279) at 1/1000 dilution + HEK 293T cells at 25 µg
Developed using the ECL technique.
Performed under reducing conditions.
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Western blot - Anti-Robo1 antibody (ab7279)All lanes : Anti-Robo1 antibody (ab7279) at 1 µg/ml
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 5 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 6 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 7 : LNCaP (human prostate cancer cell line) whole cell lysate
Lane 8 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
Lane 9 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
Lane 10 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 11 : A-172 (human brain glioblastoma) whole cell lysate
Lane 12 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 35 µg per lane.
Secondary
All lanes : Peroxidase Rabbit secondary antibody for 60 minutes at room temperature at 1/30000 dilution
Observed band size: 181 kDa why is the actual band size different from the predicted?Primary antibody incubated overnight at 4C.
Blocking Buffer: 1% Casein-TTBS for 30 min at room temperature.
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ELISA - Anti-Robo1 antibody (ab7279)ELISA results of purified ab7279 tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated secondary and TMB ELISA Peroxidase Substrate.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (54)
ab7279 は 54 報の論文で使用されています。
- Huang Z et al. Matrine suppresses liver cancer progression and the Warburg effect by regulating the circROBO1/miR-130a-5p/ROBO1 axis. J Biochem Mol Toxicol 37:e23436 (2023). PubMed: 37376914
- Kong H et al. MicroRNA-29a-3p prevents Schistosoma japonicum-induced liver fibrosis by targeting Roundabout homolog 1 in hepatic stellate cells. Parasit Vectors 16:184 (2023). PubMed: 37280619
- Cristea I et al. A Pellino-2 variant is associated with constitutive NLRP3 inflammasome activation in a family with ocular pterygium-digital keloid dysplasia. FEBS Lett 597:1290-1299 (2023). PubMed: 36776133
- Guillemot-Legris O et al. Local Administration of Minocycline Improves Nerve Regeneration in Two Rat Nerve Injury Models. Int J Mol Sci 24:N/A (2023). PubMed: 37569473
- Yin T & Zhao H miR-152-3p impedes the malignant phenotypes of hepatocellular carcinoma by repressing roundabout guidance receptor 1. Cell Mol Biol Lett 27:22 (2022). PubMed: 35236289