Anti-RNF34 抗体 (ab47255)
Key features and details
- Rabbit polyclonal to RNF34
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RNF34 antibody
RNF34 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RNF34 -
由来種
Rabbit -
特異性
A BLAST analysis was used to suggest cross-reactivity with RNF34 protein from orangutan (99%), bovine (91%), dog (89%), mouse (88%) and rat (85%) based on a high degree of protein:protein homology with the immunizing sequence. Expect reactivity with isoform 1 and 2 of RNF34. Reactivity against homologues from other sources is not known.
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog
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免疫原
Recombinant full length protein (Human)
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ポジティブ･コントロール
- RNF34 transfected lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
This protein A purified antibody is directed against human RNF34 protein. Â The product was purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the buffer. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47255の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 41 kDa.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 41 kDa. |
ターゲット情報
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機能
E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis (PubMed:15069192). May mediate 'Lys-48'-linked polyubiquitination of RIPK1 and its subsequent proteasomal degradation thereby indirectly regulating the tumor necrosis factor-mediated signaling pathway (Ref.13). Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation (PubMed:17121812). Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN (PubMed:18382127). Mediates PPARGC1A proteasomal degradation probably through ubiquitination thereby indirectly regulating the metabolism of brown fat cells (PubMed:22064484). Possibly involved in innate immunity, through 'Lys-48'-linked polyubiquitination of NOD1 and its subsequent proteasomal degradation (PubMed:25012219). -
組織特異性
Ubiquitous. Detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 1 FYVE-type zinc finger.
Contains 1 RING-type zinc finger.
Contains 2 SAP domains. -
ドメイン
The RING-type zinc finger is required for the ubiquitination of target proteins.
The FYVE-type zinc finger domain is required for localization and may confer affinity for cellular compartments enriched in phosphatidylinositol 5-phosphate and phosphatidylinositol 3-phosphate phospholipids. -
翻訳後修飾
Autoubiquitinated (in vitro).
Proteolytically cleaved by caspases upon induction of apoptosis by TNF. -
細胞内局在
Cell membrane. Endomembrane system. Nucleus. Nucleus speckle. Cytoplasm, cytosol. - Information by UniProt
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参照データベース
- Entrez Gene: 506764 Cow
- Entrez Gene: 477470 Dog
- Entrez Gene: 80196 Human
- Entrez Gene: 80751 Mouse
- Entrez Gene: 282845 Rat
- Omim: 608299 Human
- SwissProt: Q5E9J6 Cow
- SwissProt: Q969K3 Human
see all -
別名
- CARP 1 antibody
- CARP-1 antibody
- CARP1 antibody
see all
画像
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Western blot - Anti-RNF34 antibody (ab47255)All lanes : Anti-RNF34 antibody (ab47255) at 1/1000 dilution
Lane 1 : Empty vector 293T cell lysate
Lane 2 : RNF34 transfected 293T cell lysate
Lane 3 : RFFL transfected 293T cell lysate
Lysates/proteins at 20 µg/ml per lane.
Predicted band size: 41 kDa
Observed band size: 41 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab47255 は論文での使用が確認できていません。