Anti-RNF31/HOIP 抗体 (ab187976)
Key features and details
- Rabbit polyclonal to RNF31/HOIP
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RNF31/HOIP antibody
RNF31/HOIP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RNF31/HOIP -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Recombinant fragment corresponding to Human RNF31/HOIP aa 650-800.
Database link: Q96EP0 -
ポジティブ･コントロール
- IHC-P: Human kidney tissue; ICC: PC-3 whole cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187976の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
| 特記事項 |
|---|
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
ターゲット情報
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機能
E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Binds polyubiquitin of different linkage types. -
組織特異性
Expressed in both normal and transformed breast epithelial cell lines. -
配列類似性
Contains 2 IBR-type zinc fingers.
Contains 3 RanBP2-type zinc fingers.
Contains 1 RING-type zinc finger.
Contains 1 UBA domain. -
ドメイン
The RanBP2-type zinc fingers mediate the specific interaction with ubiquitin. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 55072 Human
- Entrez Gene: 268749 Mouse
- Entrez Gene: 364386 Rat
- Omim: 612487 Human
- SwissProt: Q96EP0 Human
- SwissProt: Q924T7 Mouse
- Unigene: 375217 Human
- Unigene: 434081 Human
see all -
別名
- E3 ubiquitin protein ligase RNF31 antibody
- FLJ10111 antibody
- FLJ23501 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF31/HOIP antibody (ab187976)
Immunohistochemical analysis of paraffin embedded human kidney tissue labeling RNF31/HOIP in the cytoplasm and membrane of cells in tubules with ab187976 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-RNF31/HOIP antibody (ab187976)Immunocytochemical analysis of PC-3 (Human prostate adenocarcinoma cell line) whole cells labeling RNF31/HOIP in the cytosol with ab187976 at 2 µg/ml.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab187976 は 3 報の論文で使用されています。
- Nakazawa S et al. Identification and molecular analysis of RNF31 Q622H germline polymorphism. Oncol Lett 24:394 (2022). PubMed: 36276481
- Du M et al. Liquid phase separation of NEMO induced by polyubiquitin chains activates NF-κB. Mol Cell 82:2415-2426.e5 (2022). PubMed: 35477005
- Nakayama Y et al. Linear Polyubiquitin Chain Modification of TDP-43-Positive Neuronal Cytoplasmic Inclusions in Amyotrophic Lateral Sclerosis. J Neuropathol Exp Neurol 79:256-265 (2020). PubMed: 31951008