Anti-RNF216 抗体 (ab25961)
Key features and details
- Rabbit polyclonal to RNF216
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-RNF216 antibody
RNF216 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RNF216 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide corresponding to Mouse RNF216 (N terminal).
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ポジティブ･コントロール
- Mouse heart cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Ion Exchange Chromatography -
特記事項（精製）
Purified by ion exchange chromatography -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab25961の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 10 µg/ml.
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| ICC/IF |
Use a concentration of 20 µg/ml.
|
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| WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 99 kDa).
|
| 特記事項 |
|---|
|
IHC-P
Use a concentration of 10 µg/ml. |
|
ICC/IF
Use a concentration of 20 µg/ml. |
|
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 99 kDa). |
ターゲット情報
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機能
Isoform 1 acts as an E3 ubiquitin ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Promotes degradation of TRAF3, TLR4 and TLR9. Contributes to the regulation of antiviral responses. Down-regulates activation of NF-kappa-B, IRF3 activation and IFNB production. Isoform 3 inhibits TNF and IL-1 mediated activation of NF-kappa-B. Promotes TNF and RIP mediated apoptosis. -
組織特異性
Ubiquitous, with the highest levels of expression in testis and peripheral blood leukocytes. -
パスウェイ
Protein modification; protein ubiquitination. -
関連疾患
Gordon Holmes syndrome -
配列類似性
Contains 1 IBR-type zinc finger.
Contains 2 RING-type zinc fingers. -
ドメイン
The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme. -
翻訳後修飾
Auto-ubiquitinated. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 108086 Mouse
- SwissProt: P58283 Mouse
- Unigene: 381440 Mouse
- Unigene: 488397 Mouse
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別名
- CAHH antibody
- E3 ubiquitin ligase TRIAD3 antibody
- E3 ubiquitin-protein ligase RNF216 antibody
see all
画像
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Western blot - Anti-RNF216 antibody (ab25961)Lane 1 : Anti-RNF216 antibody (ab25961) at 0.5 µg/ml
Lane 2 : Anti-RNF216 antibody (ab25961) at 1 µg/ml
Lane 3 : Anti-RNF216 antibody (ab25961) at 2 µg/ml
All lanes : Mouse heart cell lysates
Predicted band size: 99 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF216 antibody (ab25961)ab25961 at 10µg/ml staining RNF216 in mouse heart by IHC
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Immunocytochemistry/ Immunofluorescence - Anti-RNF216 antibody (ab25961)Immunofluorescence of RNF216 in Mouse Heart cells using ab25961 at 20 ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab25961 は 4 報の論文で使用されています。
- Evankovich J et al. Toll-Like Receptor 8 Stability is Regulated by Ring Finger 216 in response to circulating MicroRNAs. Am J Respir Cell Mol Biol N/A:N/A (2019). PubMed: 31385713
- Rajagopalan S et al. TNFR-associated factor 6 and TGF-ß-activated kinase 1 control signals for a senescence response by an endosomal NK cell receptor. J Immunol 192:714-21 (2014). PubMed: 24337384
- Xu C et al. Regulation of autophagy by E3 ubiquitin ligase RNF216 through BECN1 ubiquitination. Autophagy 10:2239-50 (2014). PubMed: 25484083
- Miah SM et al. Ubiquitylation of an internalized killer cell Ig-like receptor by Triad3A disrupts sustained NF-?B signaling. J Immunol 186:2959-69 (2011). PubMed: 21270397