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Anti-RNF13 抗体 - C-terminal (ab151601)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF13 antibody - C-terminal (ab151601)
  • Western blot - Anti-RNF13 antibody - C-terminal (ab151601)
  • Immunocytochemistry/ Immunofluorescence - Anti-RNF13 antibody - C-terminal (ab151601)

Key features and details

  • Rabbit polyclonal to RNF13 - C-terminal
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-RNF13 antibody - C-terminal

  • 製品の詳細

    Rabbit polyclonal to RNF13 - C-terminal

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Cow

  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 319 and 381 of RNF13 (Uniprot ID: O43567)

  • ポジティブ･コントロール

    • 293T, A431, H1299, HeLa, HepG2, Molt-4 and Raji whole cell lysate; MCF7 cells; A549 xenograft tissue

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab151601の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/500 - 1/3000. Predicted molecular weight: 43 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.
特記事項
WB
1/500 - 1/3000. Predicted molecular weight: 43 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.

ターゲット情報

  • 機能

    E3 ubiquitin-protein ligase that may play a role in controlling cell proliferation.

  • 組織特異性

    Widely expressed (at protein level). In normal pancreas, expressed in islets, but not in ducts, nor in acini (at protein level).

  • パスウェイ

    Protein modification; protein ubiquitination.

  • 配列類似性

    Contains 1 PA (protease associated) domain.
    Contains 1 RING-type zinc finger.

  • ドメイン

    The RING-type zinc finger domain is required for E3 ligase activity.

  • 翻訳後修飾

    Auto-ubiquitinated.

  • 細胞内局在

    Endoplasmic reticulum membrane. Golgi apparatus membrane. Late endosome membrane. Lysosome membrane. Nucleus inner membrane. Under certain conditions, relocalizes to recycling endosomes and to the inner nuclear membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • E3 ubiquitin-protein ligase RNF13 antibody
      • FLJ93817 antibody
      • MGC13689 antibody
      • RING finger protein 13 antibody
      • Ring zinc finger protein antibody
      • RNF 13 antibody
      • Rnf13 antibody
      • RNF13_HUMAN antibody
      • RZF antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF13 antibody - C-terminal (ab151601)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF13 antibody - C-terminal (ab151601)
      Immunohistochemical analysis of paraffin-embedded A549 xenograft, labeling RNF13 using ab151601 at 1/500 dilution.
    • Western blot - Anti-RNF13 antibody - C-terminal (ab151601)
      Western blot - Anti-RNF13 antibody - C-terminal (ab151601)
      Anti-RNF13 antibody - C-terminal (ab151601) at 1/1000 dilution + 293T whole cell lysate at 30 µg

      Predicted band size: 43 kDa



      10% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-RNF13 antibody - C-terminal (ab151601)
      Immunocytochemistry/ Immunofluorescence - Anti-RNF13 antibody - C-terminal (ab151601)
      Immunofluorescence analysis of paraformaldehyde-fixed MCF7, labeling RNF13 using ab151601 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.

    参考文献 (1)

    ab151601 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab151601 は 1 報の論文で使用されています。

    • Stamataki D  et al. Delta1 expression, cell cycle exit, and commitment to a specific secretory fate coincide within a few hours in the mouse intestinal stem cell system. PLoS One 6:e24484 (2011). IHC . PubMed: 21915337

    レビューと Q&A

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