Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) 抗体 (ab70324)

Thank you for contacting Abcam and thank you for your patience while I consulted my coleagues about your enquiry. Below is the response that I received from a colleague: "I would suggest using the non phosphorylated form of Pol2 as a positive control for ChIP. The phosophorylation state may change so detecting the protein would be more appropriate. I suppose it depends on what experiments they are doing, in this case the experimental cells may have higher phosporylation levels than the control creating artifactual results so they should be careful. They may need to do a second control to be sure." I hope this answers your questions and if I can be of anymore help, please let me know.

Please accept my apologies for misunderstanding your original email. I have to admit that I am no expert in ChIP assays but I have sent an email to ask my colleagues and I will let you know what they say. I see no reason why you could not use S2 phospho Pol2 as a positive control but as I said I am no expert. As to the discount code, under normal circumstances we have to issue the code before the original purchase but since I have already offered it to you, I will make an exception. The discount code and details of how to use it are below. Once again, please accept my apologies for the original misunderstanding and I will let you know what I hear back from my colleagues. If there is anything else I can do in the mean time, please let me know and I look forward to reading your Abreview. DISCOUNT CODE: ******** Expiration date: ******** I am very pleased to hear you would like to accept our offer and test ab70324 in ChIP. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ChIP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Thank you for contacting Abcam about ab70324. As long as there is a sufficient concentration of your sample potein present, the anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody should work. As it has not been tested in ChIP, we cannot gaurantee that it will work for you, but I can offer you our testing discount. The details of which are below: As ab70324 has not been tested in ChIP. Therefore, I can offer a discount off a future purchase if you buy ab70324 now, test it in ChIP and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab70324 in ChIP. I will then send a discount code. This code must be issued before purchasing ab70324 so please wait for my reply before ordering. 2. Purchase ab70324 either by phone, fax, or online (www.abcam.com). 3. Test it in ChIP. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab70324 turns out to be unsuitable for ChIP you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.