Anti-RNA Helicase A 抗体 [EPR13520(B)] - N-terminal (ab180189)
![Western blot - Anti-RNA Helicase A antibody [EPR13520(B)] - N-terminal (ab180189)](https://www.abcam.co.jp/ps/products/180/ab180189/Images/ab180189-208832-anti-rna-helicase-a-antibody-epr13520b-n-terminal-western-blot.jpg "Western blot - Anti-RNA Helicase A antibody [EPR13520(B)] - N-terminal (ab180189)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13520(B)] to RNA Helicase A - N-terminal
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-RNA Helicase A antibody [EPR13520(B)] - N-terminal
RNA Helicase A 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13520(B)] to RNA Helicase A - N-terminal -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, ICC/IFmore details
適用なし: IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Orangutan
-
免疫原
Synthetic peptide within Human RNA Helicase A aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q08211 -
ポジティブ･コントロール
- BxPC-3, Jurkat, HeLa and 293T cell lysates; 293T cells; Jurkat cells.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR13520(B) -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab180189の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 141 kDa.
|
|
| ICC/IF |
1/100 - 1/250.
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/5000. Predicted molecular weight: 141 kDa. |
|
ICC/IF
1/100 - 1/250. |
ターゲット情報
-
機能
Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability. -
配列類似性
Belongs to the DEAD box helicase family. DEAH subfamily.
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
ドメイン
The MTAD domain mediates interaction with the RNA polymerase II holoenzyme. The NTD domain is necessary and sufficient for nucleo-cytoplasmic shuttling and interaction with HRMT1L2 and SMN1. -
翻訳後修飾
Methylated. HRMT1L2 mediated methylation of undefined Arg residues in the NTD is required for nuclear localization.
May be phosphorylated by PRKDC/XRCC7. Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm. - Information by UniProt
-
参照データベース
- Entrez Gene: 1660 Human
- Omim: 603115 Human
- SwissProt: Q08211 Human
- SwissProt: Q5R874 Orangutan
- Unigene: 191518 Human
-
別名
- ATP dependent RNA helicase A antibody
- ATP-dependent RNA helicase A antibody
- DDX 9 antibody
see all
画像
-
Western blot - Anti-RNA Helicase A antibody [EPR13520(B)] - N-terminal (ab180189)All lanes : Anti-RNA Helicase A antibody [EPR13520(B)] - N-terminal (ab180189) at 1/1000 dilution
Lane 1 : BxPC-3 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 141 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-RNA Helicase A antibody [EPR13520(B)] - N-terminal (ab180189)](https://www.abcam.co.jp/ps/products/180/ab180189/Images/ab180189-208834-anti-rna-helicase-a-antibody-epr13520b-n-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RNA Helicase A antibody [EPR13520(B)] - N-terminal (ab180189)Immunofluorescent analysis of 293T cells labeling RNA Helicase A with ab180189 at 1/100.
-
![Flow Cytometry (Intracellular) - Anti-RNA Helicase A antibody [EPR13520(B)] - N-terminal (ab180189)](https://www.abcam.co.jp/ps/products/180/ab180189/Images/ab180189-208835-anti-rna-helicase-a-antibody-epr13520b-n-terminal-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-RNA Helicase A antibody [EPR13520(B)] - N-terminal (ab180189)Intracellular flow cytometrical analysis of permeabilized Jurkat cells labeling RNA Helicase A with ab180189 at 1/10 (red) or a rabbit IgG negative (green).
-
![Anti-RNA Helicase A antibody [EPR13520(B)] - N-terminal (ab180189)](https://www.abcam.co.jp/ps/products/180/ab180189/Images/ab180189-4-benefits-of-recombinant-antibodies.png)
Anti-RNA Helicase A antibody [EPR13520(B)] - N-terminal (ab180189)
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab180189 は論文での使用が確認できていません。