Anti-RIP140 抗体 [2656C6a] (ab53740)
![Western blot - Anti-RIP140 antibody [2656C6a] (ab53740)](https://www.abcam.co.jp/ps/products/53/ab53740/Images/rip140.jpg "Western blot - Anti-RIP140 antibody [2656C6a] (ab53740)")
Key features and details
- Mouse monoclonal [2656C6a] to RIP140
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
製品の概要
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製品名
Anti-RIP140 antibody [2656C6a]
RIP140 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2656C6a] to RIP140 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Recombinant fragment -
免疫原
Recombinant fragment (Human)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. -
ポリ/モノ
モノクローナル -
クローン名
2656C6a -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53740の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 129 kDa.
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| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 129 kDa. |
ターゲット情報
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機能
Modulates transcriptional activation by steroid receptors such as NR3C1, NR3C2 and ESR1. Also modulates transcriptional repression by nuclear hormone receptors. -
ドメイン
Contains 9 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, which have different affinities for nuclear receptors. The C-terminal LTKTNPILYYMLQK motif is required for ligand-dependent interaction with RAAR and RXRB homodimers and heterodimers, for the corepressor activity, and for the formation of an HDAC3 complex with RARA/RXRB (By similarity). Contains at least four autonomous repression domains (RD1-4). RD1 functions via a histone deacetylase (HDAC)-independent mechanism, whereas RD2, RD3 and RD4 can function by HDAC-dependent or independent mechanisms, depending on cell type. RD2 is dependent on CTBP binding. -
翻訳後修飾
Acetylation regulates its nuclear translocation and corepressive activity (By similarity). Acetylation abolishes interaction with CTBP1. Phosphorylation enhances interaction with YWHAH. -
細胞内局在
Nucleus. Localized to discrete foci and redistributes to larger nuclear domains upon binding to ligand-bound NR3C1. - Information by UniProt
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別名
- NRIP 1 antibody
- NRIP1 antibody
- NRIP1_HUMAN antibody
see all
画像
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Western blot - Anti-RIP140 antibody [2656C6a] (ab53740)Anti-RIP140 antibody [2656C6a] (ab53740) + Immunizing recombinant protein
Predicted band size: 129 kDa
Observed band size: 41 kDa why is the actual band size different from the predicted?
The band size does not correspond to the predicted protein size, as the recombinant fragment immunogen was used for the test lane
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab53740 は 1 報の論文で使用されています。
- Ni XF et al. MicroRNA-548-3p and MicroRNA-576-5p enhance the migration and invasion of esophageal squamous cell carcinoma cells via NRIP1 down-regulation. Neoplasma 65:881-887 (2018). PubMed: 29940757