Anti-RING2 / RING1B / RNF2 抗体 (ab3832)
Key features and details
- Goat polyclonal to RING2 / RING1B / RNF2
- Suitable for: WB
- Reacts with: Mouse, Human, Chinese hamster
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-RING2 / RING1B / RNF2 antibody
RING2 / RING1B / RNF2 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to RING2 / RING1B / RNF2 -
由来種
Goat -
特異性
Affinity purified Anti-RING1B antibody is directed against human RING1B protein. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, orangutan, mouse, rat, dog, bovine, frog and chicken based on 100% homology for the immunogen sequence. Expect cross reactivity with RING1B from zebrafish, as only a single amino acid residue changes within the immunogen sequence (92% positive by BLAST). Cross reactivity with RING1B homologues from other sources has not been determined.
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human, Chinese hamster -
免疫原
Synthetic peptide:
EAGPSNKRTKTSDC
, corresponding to amino acids 189-201 of Human RING1B. -
特記事項
RING1B interacts with CP2, a mammalian member of the grainyhead-like family of transcription factors, in vitro and in vivo. The functional consequence of this interaction is repression of CP2-dependent transcription.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Affinity purified Anti-RING1B antibody is directed against human RING1B protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. -
一次抗体 備考
RING1B interacts with CP2, a mammalian member of the grainyhead-like family of transcription factors, in vitro and in vivo. The functional consequence of this interaction is repression of CP2-dependent transcription. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3832の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/500 - 1/2000. Detects a band of approximately 43 kDa (predicted molecular weight: 37.6 kDa).
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特記事項 |
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WB
1/500 - 1/2000. Detects a band of approximately 43 kDa (predicted molecular weight: 37.6 kDa). |
ターゲット情報
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機能
E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 1 RING-type zinc finger. -
翻訳後修飾
Polyubiquitinated in the presence of UBE2D3 (in vitro).
Monoubiquitinated, by auto-ubiquitination. -
細胞内局在
Nucleus. Chromosome. Enriched on inactive X chromosome (Xi) in female trophoblast stem (TS) cells as well as differentiating embryonic stem (ES) cells. The enrichment on Xi is transient during TS and ES cell differentiation. The association with Xi is mitotically stable in non-differentiated TS cells. - Information by UniProt
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参照データベース
- Entrez Gene: 6045 Human
- Entrez Gene: 19821 Mouse
- Omim: 608985 Human
- SwissProt: Q99496 Human
- SwissProt: Q9CQJ4 Mouse
- Unigene: 591490 Human
- Unigene: 482250 Mouse
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別名
- BAP 1 antibody
- BAP1 antibody
- DING antibody
see all
画像
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Western blot using ab3832 at 1/500. Lysates at 20
Western blot using ab3832 at 1/500. Lysates at 20µg/lane. g/lane.µ
Lane 1: 3T3 cell lysate
Lane 2: U937 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Mouse brain lysate
Lane 5: CHO-K1 cell lysate
Secondary ab: rabbit polyclonal to goat IgG ab6741 1/5000
Exposure time: 3 minutes
Lane 2: U937 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Mouse brain lysate
Lane 5: CHO-K1 cell lysate
Secondary ab: rabbit polyclonal to goat IgG ab6741 1/5000 Exposure time: 3 minutes
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (25)
ab3832 は 25 報の論文で使用されています。
- Sawai A et al. PRC1 sustains the integrity of neural fate in the absence of PRC2 function. Elife 11:N/A (2022). PubMed: 34994686
- Naik NG et al. KDM2B Overexpression Facilitates Lytic De Novo KSHV Infection by Inducing AP-1 Activity Through Interaction with the SCF E3 Ubiquitin Ligase Complex. J Virol 95:N/A (2021). PubMed: 33692209
- Plys AJ et al. Phase separation of Polycomb-repressive complex 1 is governed by a charged disordered region of CBX2. Genes Dev 33:799-813 (2019). PubMed: 31171700
- Papp B et al. Genome-wide identification of direct RTA targets reveals key host factors for KSHV lytic reactivation. J Virol N/A:N/A (2018). PubMed: 30541837
- Rothberg JLM et al. Mtf2-PRC2 control of canonical Wnt signaling is required for definitive erythropoiesis. Cell Discov 4:21 (2018). PubMed: 29736258