Anti-RING2 / RING1B / RNF2 抗体 (ab187509)
Key features and details
- Rabbit polyclonal to RING2 / RING1B / RNF2
- Suitable for: ChIP, IP, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RING2 / RING1B / RNF2 antibody
RING2 / RING1B / RNF2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RING2 / RING1B / RNF2 -
由来種
Rabbit -
アプリケーション
適用あり: ChIP, IP, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant full length protein corresponding to Human RING2/ RING1B/ RNF2 aa 1 to the C-terminus.
Database link: Q99496 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187509の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ChIP |
1/50 - 1/200.
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| IP |
1/50 - 1/200.
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| WB |
1/500 - 1/2000. Predicted molecular weight: 37 kDa.
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| IHC-P |
1/50 - 1/200.
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| 特記事項 |
|---|
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ChIP
1/50 - 1/200. |
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IP
1/50 - 1/200. |
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WB
1/500 - 1/2000. Predicted molecular weight: 37 kDa. |
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IHC-P
1/50 - 1/200. |
ターゲット情報
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機能
E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 1 RING-type zinc finger. -
翻訳後修飾
Polyubiquitinated in the presence of UBE2D3 (in vitro).
Monoubiquitinated, by auto-ubiquitination. -
細胞内局在
Nucleus. Chromosome. Enriched on inactive X chromosome (Xi) in female trophoblast stem (TS) cells as well as differentiating embryonic stem (ES) cells. The enrichment on Xi is transient during TS and ES cell differentiation. The association with Xi is mitotically stable in non-differentiated TS cells. - Information by UniProt
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参照データベース
- Entrez Gene: 6045 Human
- Entrez Gene: 19821 Mouse
- Entrez Gene: 304850 Rat
- Omim: 608985 Human
- SwissProt: Q99496 Human
- SwissProt: Q9CQJ4 Mouse
- SwissProt: Q4KLY4 Rat
- Unigene: 591490 Human
see all -
別名
- BAP 1 antibody
- BAP1 antibody
- DING antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING2 / RING1B / RNF2 antibody (ab187509)
Paraffin-embedded human gastric cancer tissue stained for RING2 / RING1B / RNF2 using ab187509 at 1/100 dilution in immunohistochemical analysis.
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Western blot - Anti-RING2 / RING1B / RNF2 antibody (ab187509)All lanes : Anti-RING2 / RING1B / RNF2 antibody (ab187509) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : U-937 cell lysate
Lane 4 : SH-SY5Y cell lysate
Lane 5 : Mouse brain tissue lysate
Lane 6 : Mouse spleen tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 90 secondsBlocking buffer: 3% nonfat dry milk in TBST.
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Immunoprecipitation - Anti-RING2 / RING1B / RNF2 antibody (ab187509)Immunoprecipitation analysis of 200μg extracts of 293T cells using 1μg of ab187509. Western blot was performed from the immunoprecipitate using ab187509 at a dilution of 1:1000.
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ChIP - Anti-RING2 / RING1B / RNF2 antibody (ab187509)Chromatin immunoprecipitation analysis of extracts of HeLa cells, using ab187509 and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING2 / RING1B / RNF2 antibody (ab187509)Paraffin-embedded mouse brain tissue stained for RING2 / RING1B / RNF2 using ab187509 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING2 / RING1B / RNF2 antibody (ab187509)Paraffin-embedded rat lung tissue stained for RING2 / RING1B / RNF2 using ab187509 at 1/100 dilution in immunohistochemical analysis.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab187509 は 1 報の論文で使用されています。
- Boulay G et al. The chromatin landscape of primary synovial sarcoma organoids is linked to specific epigenetic mechanisms and dependencies. Life Sci Alliance 4:N/A (2021). PubMed: 33361335