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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-RING1 抗体 [EPR13047] (ab180170)

Submit a review Submit a question References (1)
Immunoprecipitation - Anti-RING1 antibody [EPR13047] (ab180170)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [EPR13047] (ab180170)
  • Western blot - Anti-RING1 antibody [EPR13047] (ab180170)
  • Western blot - Anti-RING1 antibody [EPR13047] (ab180170)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [EPR13047] (ab180170)
  • Immunocytochemistry/ Immunofluorescence - Anti-RING1 antibody [EPR13047] (ab180170)
  • Anti-RING1 antibody [EPR13047] (ab180170)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13047] to RING1
  • Suitable for: IHC-P, WB, ICC/IF, IP
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-RING1 antibody [EPR13047]
    RING1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13047] to RING1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WB, ICC/IF, IPmore details
    適用なし: Flow Cyt

  • 種交差性

    交差種: Human
    交差が予測される動物種: Dog, Chimpanzee, Rhesus monkey, Gorilla

  • 免疫原

    Synthetic peptide within Human RING1 aa 150-250 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q06587

  • ポジティブ･コントロール

    • Human endometrial carcinoma and pancreas tissues. Ramos, MOLT4, LNCaP and HeLa whole cell lysate (ab150035). HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR13047

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab180170の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
ICC/IF
1/50 - 1/100.
IP
1/30.
特記事項
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
ICC/IF
1/50 - 1/100.
IP
1/30.
追加情報
Is unsuitable for Flow Cyt.

ターゲット情報

  • 機能

    Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.

  • パスウェイ

    Protein modification; protein ubiquitination.

  • 配列類似性

    Contains 1 RING-type zinc finger.

  • 細胞内局在

    Nucleus speckle.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Ring1A antibody
    • E3 ubiquitin-protein ligase RING1 antibody
    • Polycomb complex protein RING1 antibody
    • Really interesting new gene 1 protein antibody
    • RING finger protein 1 antibody
    • Ring1 antibody
    • RING1_HUMAN antibody
    • RING1A antibody
    • Rnf1 antibody
    • Transcription repressor Ring1A antibody
    see all

画像

  • Immunoprecipitation - Anti-RING1 antibody [EPR13047] (ab180170)
    Immunoprecipitation - Anti-RING1 antibody [EPR13047] (ab180170)

    RING1 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab180170 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab180170 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/10000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug

    Lane 2: ab180170 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab180170 in mouse cerebellum tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [EPR13047] (ab180170)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [EPR13047] (ab180170)

    Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling RING1 using ab180170 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-RING1 antibody [EPR13047] (ab180170)
    Western blot - Anti-RING1 antibody [EPR13047] (ab180170)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: RING1 knockout HAP1 cell lysate (20 µg)
    Lane 3: MOLT4 cell lysate (20 µg)
    Lane 4: LnCaP cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab180170 observed at 52 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab180170 was shown to specifically react with RING1 when RING1 knockout samples were used. Wild-type and RING1 knockout samples were subjected to SDS-PAGE. ab180170 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-RING1 antibody [EPR13047] (ab180170)
    Western blot - Anti-RING1 antibody [EPR13047] (ab180170)
    All lanes : Anti-RING1 antibody [EPR13047] (ab180170) at 1/5000 dilution

    Lane 1 : Ramos (human Burkitt's lymphoma) whole cell lysate
    Lane 2 : MOLT-4 (human acute lymphoblastic leukemia) whole cell lysate
    Lane 3 : LNCaP (Human prostate carcinoma) whole cell lysate
    Lane 4 : HeLa (human cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution

    Predicted band size: 42 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration 5% NFDM/TBST.

    Exposure Time:
    Lane 1-3: 3 minutes;
    Lane 4: 1 minute

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [EPR13047] (ab180170)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [EPR13047] (ab180170)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling RING1 using ab180170 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-RING1 antibody [EPR13047] (ab180170)
    Immunocytochemistry/ Immunofluorescence - Anti-RING1 antibody [EPR13047] (ab180170)

    Immunofluorescence analysis of HeLa cells labeling RING1 using ab180170 at 1/50 dilution.

  • Anti-RING1 antibody [EPR13047] (ab180170)
    Anti-RING1 antibody [EPR13047] (ab180170)

参考文献 (1)

ab180170 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab180170 は 1 報の論文で使用されています。

  • Maat H  et al. The USP7-TRIM27 axis mediates non-canonical PRC1.1 function and is a druggable target in leukemia. iScience 24:102435 (2021). PubMed: 34113809

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