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Anti-RIM2 抗体 (ab69860)

Reviews (1)Q&A (3)References (1)
Western blot - Anti-RIM2 antibody (ab69860)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIM2 antibody (ab69860)

Key features and details

  • Rabbit polyclonal to RIM2
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-RIM2 antibody

  • 製品の詳細

    Rabbit polyclonal to RIM2

  • 由来種

    Rabbit

  • 特異性

    RIM2 is known to exist in multiple isoforms; this antibody should recognize most of them. The antibody is predicted to have no cross reactivity to other RIM proteins.

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: IHC-FoFr

  • 種交差性

    交差種: Human

  • 免疫原

    A 17 amino acid synthetic peptide near the center of human RIM2

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab69860の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 160 kDa.
IHC-P
Use a concentration of 5 µg/ml.
特記事項
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 160 kDa.
IHC-P
Use a concentration of 5 µg/ml.
追加情報
Is unsuitable for IHC-FoFr.

ターゲット情報

  • 機能

    Rab effector involved in exocytosis. May act as scaffold protein.

  • 配列類似性

    Contains 2 C2 domains.
    Contains 1 FYVE-type zinc finger.
    Contains 1 PDZ (DHR) domain.
    Contains 1 RabBD (Rab-binding) domain.

  • 細胞内局在

    Cell membrane. Cell junction > synapse. Cell junction > synapse > presynaptic cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • KIAA0751 antibody
      • Non small cell lung cancer RimL3a protein antibody
      • Non small cell lung cancer RimL3c protein antibody
      • OBOE antibody
      • Protein regulating synaptic membrane exocytosis 2 antibody
      • Rab-3-interacting molecule 2 antibody
      • Rab-3-interacting protein 3 antibody
      • Rab3 interacting molecule 2 antibody
      • RAB3IP3 antibody
      • Regulating synaptic membrane exocytosis 2 antibody
      • Regulating synaptic membrane exocytosis protein 2 antibody
      • RIM 2 antibody
      • Rims2 (gene name) antibody
      • Rims2 antibody
      • RIMS2_HUMAN antibody
      see all

    画像

    • Western blot - Anti-RIM2 antibody (ab69860)
      Western blot - Anti-RIM2 antibody (ab69860)
      Anti-RIM2 antibody (ab69860) at 1 µg/ml + Human brain lysates

      Predicted band size: 160 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIM2 antibody (ab69860)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIM2 antibody (ab69860)

      ab69860 at 5 µg/ml staining RIM2 in human prostate tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

    参考文献 (1)

    ab69860 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab69860 は 1 報の論文で使用されています。

    • Park JH  et al. Glucagon-like peptide-1 enhances glucokinase activity in pancreatic ß-cells through the association of Epac2 with Rim2 and Rab3A. Endocrinology 153:574-82 (2012). WB ; Human . PubMed: 22147008

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-RIM2 antibody

     Below Average
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Cortex)
    Specification
    Cortex
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Karine Thibault

    Verified customer

    投稿 Nov 08 2010

    Question

    Band around 62 kDa band in WB with mouse brain lysates, pre and post-IP.

    Read More
    Answer



    As we discussed, I'd recommend pre-clearing the lysates with normal rabbit serum to remove anything in the lysate that binds non-specifically with IgG. You can also look through the literature to see if other researchers have reported an isoform of RIM2 around 62 kDa.

    Read More

    Question

    Hello,



    I have a question regarding the following AB: ab69860



    What is the epitope of it? Would it recognize human recombinant RIM 2 aa. 181-261?



    Thx!

    Read More
    Answer

    Thank you for your inquiry.

    I have to confirm that the exact immunogen sequence of ab69860is proprietary information, but it is from within amino acids 201-260 of human RIM2.

    Therefore this antibody should be suitable for a recombinant human RIM2 from aa 181-261, since the immunogen is included in this sequence.

    I hope this information is helpful. Please do not hesitate to contact me again with any further questions.

    Read More

    Question

    What other isoforms can this product detect?

    Read More
    Answer

    Thank you for your enquiry. This product detects isoform 4, which is predicted to be about 91.9kDa in size. Based on the immunogen sequence, this antibody will cross-react with human isoforms 2 through 6 of human RIM2, and mice and rats should have similar isoforms. 1. Human protein: IPI00005698 * 135250 Da * RIMS2 Isoform 1 2. Human protein: IPI00249249 * 160403 Da * RIMS2 Isoform 6 3. Human protein: IPI00339347 * 119057 Da * RIMS2 Isoform 2 4. Human protein: IPI00339350 * 94172 Da * RIMS2 Isoform 5 5. Human protein: IPI00396227 * 91941 Da * RIMS2 Isoform 4 6. Human protein: IPI00398789 * 30972 Da * RIMS2 Isoform 7 7. Human protein: IPI00873023 * 157822 Da * RIMS2 Isoform 3 Please let me know if you have any more questions.

    Read More

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