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リコンビナントRabMAb

Anti-RIG-I/DDX58 抗体 [EPR26465-40] (ab302778)

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Western blot - Anti-RIG-I/DDX58 antibody [EPR26465-40] (AB302778)
  • Western blot - Anti-RIG-I/DDX58 antibody [EPR26465-40] (AB302778)
  • Immunocytochemistry/ Immunofluorescence - Anti-RIG-I/DDX58 antibody [EPR26465-40] (AB302778)
  • Immunoprecipitation - Anti-RIG-I/DDX58 antibody [EPR26465-40] (AB302778)
  • Anti-RIG-I/DDX58 antibody [EPR26465-40] (AB302778)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26465-40] to RIG-I/DDX58
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-RIG-I/DDX58 antibody [EPR26465-40]
    RIG-I/DDX58 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26465-40] to RIG-I/DDX58

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IF, IPmore details
    適用なし: Flow Cyt (Intra) or IHC-P

  • 種交差性

    交差種: Mouse
    非交差種: Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: RAW 264.7 whole cell lysate, treated or non-treated with LPS. NIH/3T3 whole cell lysate. Mouse spleen and pancreas tissue lysate. ICC/IF: LPS treated RAW 264.7 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302778の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 102,80 kDa (predicted molecular weight: 107 kDa).
ICC/IF
1/500.
IP
1/30.
特記事項
WB
1/1000. Detects a band of approximately 102,80 kDa (predicted molecular weight: 107 kDa).
ICC/IF
1/500.
IP
1/30.
追加情報
Is unsuitable for Flow Cyt (Intra) or IHC-P.

ターゲット情報

  • 機能

    Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). Essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV.

  • 組織特異性

    Present in vascular smooth cells (at protein level).

  • 配列類似性

    Belongs to the helicase family.
    Contains 2 CARD domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.

  • ドメイン

    The repressor domain controls homomultimerization and interaction with MAVS.
    The helicase domain is responsible for dsRNA recognition.
    The 2 CARD domains are responsible for interaction with and signaling through MAVS.
    The second CARD domain is the primary site for 'Lys-63'-linked ubiquitination.

  • 翻訳後修飾

    Isgylated. Conjugated to ubiquitin-like protein ISG15 upon IFN-beta stimulation.
    Ubiquitinated. Undergoes 'Lys-63'-linked ubiquitination. Lys-172 is the critical site for TRIM25-mediated ubiquitination, for MAVS binding and to induce anti-viral signal transduction. Lys-154, Lys-164 and Lys-172 are critical sites for RNF135-mediated ubiquitination. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains.

  • 細胞内局在

    Cytoplasm. Colocalized with TRIM25 at cytoplasmic perinuclear bodies.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Ddx58 antibody
      • DDX58_HUMAN antibody
      • DEAD (Asp Glu Ala Asp) box polypeptide 58 antibody
      • DEAD (Asp Glu Ala Asp/His) box polypeptide antibody
      • DEAD box protein 58 antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide RIG1 antibody
      • DKFZp434J1111 antibody
      • DKFZp686N19181 antibody
      • FLJ13599 antibody
      • Probable ATP dependent RNA helicase DDX58 antibody
      • Probable ATP-dependent RNA helicase DDX58 antibody
      • Retinoic acid inducible gene 1 protein antibody
      • Retinoic acid-inducible gene 1 protein antibody
      • Retinoic acid-inducible gene I protein antibody
      • RIG I antibody
      • Rig-1 antibody
      • RIG-I antibody
      • RIG1 antibody
      • rigi antibody
      • RLR 1 antibody
      • RNA helicase antibody
      • RNA helicase RIG I antibody
      • SGMRT2 antibody
      see all

    画像

    • Western blot - Anti-RIG-I/DDX58 antibody [EPR26465-40] (AB302778)
      Western blot - Anti-RIG-I/DDX58 antibody [EPR26465-40] (AB302778)
      All lanes : Anti-RIG-I/DDX58 antibody [EPR26465-40] (ab302778) at 1/1000 dilution

      Lane 1 : Untreated RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
      Lane 2 : RAW 264.7 treated with 1ug/ml LPS (lipopolysaccharide) for 16 hours, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 107 kDa
      Observed band size: 102, 80 kDa why is the actual band size different from the predicted?


      Exposure time: 26 seconds


      Blocking and dilution buffer: 5% NFDM/TBST. 

      The expression of RIG-I/DDX58 is upregulated in response to LPS treatment (PMID: 18523264).

      The expression molecular weight observed is consistent with what has been described in the literature (PMID: 29346611).

    • Western blot - Anti-RIG-I/DDX58 antibody [EPR26465-40] (AB302778)
      Western blot - Anti-RIG-I/DDX58 antibody [EPR26465-40] (AB302778)
      All lanes : Anti-RIG-I/DDX58 antibody [EPR26465-40] (ab302778) at 1/1000 dilution

      Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
      Lane 2 : Mouse spleen tissue lysate
      Lane 3 : Mouse pancreas tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 107 kDa
      Observed band size: 102, 80 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-RIG-I/DDX58 antibody [EPR26465-40] (AB302778)
      Immunocytochemistry/ Immunofluorescence - Anti-RIG-I/DDX58 antibody [EPR26465-40] (AB302778)

      Immunocytochemical/Immunofluorescence analysis of 4% paraformaldehyde fixed, and 0.1% Triton X-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling RIG-I/DDX58 with ab302778 at 1/500 dilution (1.082 μg/ml), followed by preabsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)  at 1/1000 dilution (2 μg/mL)(Green). Confocal image showing increased cytoplasmic staining in RAW 264.7 cells treated with lipopolysaccharide (1 μg/mL) for 16 h. Counterstained with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (2.5 μg/ml) (Red). 

    • Immunoprecipitation - Anti-RIG-I/DDX58 antibody [EPR26465-40] (AB302778)
      Immunoprecipitation - Anti-RIG-I/DDX58 antibody [EPR26465-40] (AB302778)

      RIG-I/DDX58 was immunoprecipitated from 0.35 mg RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with AB302778 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using AB302778 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: RAW 264.7 whole cell lysate 10 μg

      Lane 2: AB302778 IP in RAW264.7 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of AB302778 in RAW264.7 whole cell lysate

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 128 seconds. 

      Observed MW (kDa): 102, 80.  

    • Anti-RIG-I/DDX58 antibody [EPR26465-40] (AB302778)
      Anti-RIG-I/DDX58 antibody [EPR26465-40] (AB302778)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302778 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302778 は論文での使用が確認できていません。

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