Anti-Rif1 抗体 (ab70254)
Key features and details
- Rabbit polyclonal to Rif1
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Rif1 antibody
Rif1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Rif1 -
由来種
Rabbit -
特異性
Reactivity with mouse Rif1 is not expected owing to divergence of sequence between this ortholog and human Rif1 at the site to which the epitope maps. -
アプリケーション
適用あり: IHC-P, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Orangutan
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免疫原
Synthetic peptide corresponding to a region between residues 1 and 50 of human Rif1 (NP_060621.3)
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ポジティブ･コントロール
- Whole cell lysate from 293T cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70254の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
Use at 1-5 µg/mg of lysate.
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| 特記事項 |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
Use at 1-5 µg/mg of lysate. |
ターゲット情報
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機能
Required for checkpoint mediated arrest of cell cycle progression in response to DNA damage during S-phase (the intra-S-phase checkpoint). This checkpoint requires activation of at least 2 parallel pathways by the ATM kinase: one involves the MRN (MRE11A-RAD50-NBS1) complex, while the second requires CHEK2. RIF1 seems to act independently of both these pathways. Seems to play no role in either the G1/S or G2/M DNA damage checkpoints. -
組織特異性
Highly expressed in testis. -
発生段階
Expression peaks in late G2/S phase of the cell cycle. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. Chromosome > telomere. Cytoplasm > cytoskeleton > spindle. Exhibits ATM- and TP53BP1-dependent localization to uncapped or aberrant telomeres and to DNA double strand breaks (DSBs). Does not associate with normal telomere structures. Localizes to microtubules of the midzone of the mitotic spindle during anaphase, and to condensed chromosomes in telophase. - Information by UniProt
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参照データベース
- Entrez Gene: 459657 Chimpanzee
- Entrez Gene: 55183 Human
- Omim: 608952 Human
- SwissProt: Q5UIP0 Human
- Unigene: 655671 Human
- Unigene: 725538 Human
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別名
- DKFZp781N1478 antibody
- FLJ10599 antibody
- FLJ11269 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rif1 antibody (ab70254)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human laryngeal squamous cell carcinoma tissue labelling Rif1 with ab70254 at 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-Rif1 antibody (ab70254)Immunoprecipitation analysis of 293T whole cell lysate (1mg for IP, 25% of reaction loaded), using ab70254 at 5 µg/mg lysate for IP. Immunoprecipitated Rif1 was blotted using ab70307 at 0.4µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab70254 は 2 報の論文で使用されています。
- Hiraga SI et al. Human RIF1 and protein phosphatase 1 stimulate DNA replication origin licensing but suppress origin activation. EMBO Rep 18:403-419 (2017). IP ; Human . PubMed: 28077461
- Alver RC et al. Reversal of DDK-Mediated MCM Phosphorylation by Rif1-PP1 Regulates Replication Initiation and Replisome Stability Independently of ATR/Chk1. Cell Rep 18:2508-2520 (2017). PubMed: 28273463