Anti-Rif1 抗体 (ab13422)
Key features and details
- Rabbit polyclonal to Rif1
- Suitable for: IP, ELISA, WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-Rif1 antibody
Rif1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Rif1 -
由来種
Rabbit -
特異性
The immunogen sequence for ab13422 represents the C-terminal end of mouse Rif1, mouse teleomere-associated protein RIF1 and mouse Rap1-interacting factor.
This affinity purified antibody is directed against mouse Rif1 protein. A BLAST analysis was used to suggest reactivity with this protein from mouse based on 100% homology for the immunogen sequence. Expect cross reactivity with Rif1 from human, chimpanzee, rat and dog as only a single amino acid residue change (92% homology) is found for the immunogen sequence. Cross reactivity with Rif1 homologues from other sources has not been determined. Databases confirm that the immunogen sequence represents the C-terminal end of mouse Rif1 (534 aa 58.1 kDa), mouse telomere-associated protein RIF1 (2418 aa 265.7 kDa) and mouse Rap1-interacting factor 1 (2426 267.1 kDa).
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アプリケーション
適用あり: IP, ELISA, WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat, Cow, Dog, Human -
免疫原
Synthetic peptide corresponding to Mouse Rif1 aa 2405-2419 (C terminal) conjugated to keyhole limpet haemocyanin.
Sequence:C-RNLQSRWRSPAHENS
Database link: Q6PR54 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This affinity purified antibody is directed against mouse Rif1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13422の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP | ||
ELISA | ||
WB | (1) |
特記事項 |
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IP: Use at an assay dependent dilution (PMID 19000314).
WB: 1/500 - 1/3000. Detects a band of approximately 265 kDa (predicted molecular weight: 265 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Required for checkpoint mediated arrest of cell cycle progression in response to DNA damage during S-phase (the intra-S-phase checkpoint). This checkpoint requires activation of at least 2 parallel pathways by the ATM kinase: one involves the MRN (MRE11A-RAD50-NBS1) complex, while the second requires CHEK2. RIF1 seems to act independently of both these pathways. Seems to play no role in either the G1/S or G2/M DNA damage checkpoints. -
組織特異性
Highly expressed in testis. -
発生段階
Expression peaks in late G2/S phase of the cell cycle. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. Chromosome > telomere. Cytoplasm > cytoskeleton > spindle. Exhibits ATM- and TP53BP1-dependent localization to uncapped or aberrant telomeres and to DNA double strand breaks (DSBs). Does not associate with normal telomere structures. Localizes to microtubules of the midzone of the mitotic spindle during anaphase, and to condensed chromosomes in telophase. - Information by UniProt
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参照データベース
- Entrez Gene: 541247 Cow
- Entrez Gene: 609234 Dog
- Entrez Gene: 55183 Human
- Entrez Gene: 51869 Mouse
- Entrez Gene: 295602 Rat
- Omim: 608952 Human
- SwissProt: Q5UIP0 Human
- SwissProt: Q6PR54 Mouse
see all -
別名
- DKFZp781N1478 antibody
- FLJ10599 antibody
- FLJ11269 antibody
see all
画像
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Anti-Rif1 antibody (ab13422) at 1 µg/ml + 25 ug of MEF whole cell lysate
Secondary
IRDye TM800 conjugated goat anti rabbit IgG (H&L) incubated for 45 minutes at 1/10000 dilution
Predicted band size: 265 kDa
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab13422 は 9 報の論文で使用されています。
- Wu C et al. circACTG1 Promotes Hepatocellular Carcinoma Progression by Regulating miR-940/RIF1 Axis and Activating AKT/mTOR Pathway. J Immunol Res 2022:8649386 (2022). PubMed: 35769514
- Li L et al. Rif1 interacts with non-canonical polycomb repressive complex PRC1.6 to regulate mouse embryonic stem cells fate potential. Cell Regen 11:25 (2022). PubMed: 35915272
- Chen BR et al. LIN37-DREAM prevents DNA end resection and homologous recombination at DNA double-strand breaks in quiescent cells. Elife 10:N/A (2021). PubMed: 34477552
- Yang D et al. REV7 is required for processing AID initiated DNA lesions in activated B cells. Nat Commun 11:2812 (2020). PubMed: 32499490
- Rao SR et al. The Clathrin-dependent Spindle Proteome. Mol Cell Proteomics 15:2537-53 (2016). PubMed: 27174698
- Dan J et al. Rif1 maintains telomere length homeostasis of ESCs by mediating heterochromatin silencing. Dev Cell 29:7-19 (2014). PubMed: 24735877
- Daxinger L et al. An ENU mutagenesis screen identifies novel and known genes involved in epigenetic processes in the mouse. Genome Biol 14:R96 (2013). PubMed: 24025402
- Cooper CD et al. Identification and Characterization of Peripheral T-Cell Lymphoma-Associated SEREX Antigens. PLoS One 6:e23916 (2011). WB ; Human . PubMed: 21887344
- Moorhead GB et al. Displacement affinity chromatography of protein phosphatase one (PP1) complexes. BMC Biochem 9:28 (2008). WB, IP ; Human . PubMed: 19000314