Anti-RICTOR 抗体 (ab70374)
Key features and details
- Rabbit polyclonal to RICTOR
- Suitable for: WB, IP, Flow Cyt, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RICTOR antibody
RICTOR 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RICTOR -
由来種
Rabbit -
特異性
Initial tests for reactivity with mouse RICTOR from NIH3T3 cells suggest that higher amounts of lysate and less dilution of ab70374 are required for brief exposure times. -
アプリケーション
適用あり: WB, IP, Flow Cyt, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to a region between residue 1650 and the C-terminus (residue 1708) of human RICTOR.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70374の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 192 kDa (predicted molecular weight: 192 kDa).
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| IP |
Use at 2-10 µg/mg of lysate.
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| Flow Cyt |
Use at an assay dependent concentration.
0.5 ug per 1 million cells in a 150 mcl reaction. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-P |
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 192 kDa (predicted molecular weight: 192 kDa). |
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IP
Use at 2-10 µg/mg of lysate. |
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Flow Cyt
Use at an assay dependent concentration. 0.5 ug per 1 million cells in a 150 mcl reaction. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development. -
配列類似性
Belongs to the RICTOR family. -
翻訳後修飾
Phosphorylated by MTOR; when part of mTORC2. Phosphorylated at Thr-1135 by RPS6KB1; phosphorylation of RICTOR inhibits mTORC2 and AKT1 signaling. - Information by UniProt
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参照データベース
- Entrez Gene: 461907 Chimpanzee
- Entrez Gene: 479356 Dog
- Entrez Gene: 101132782 Gorilla
- Entrez Gene: 253260 Human
- Entrez Gene: 78757 Mouse
- Entrez Gene: 100443248 Orangutan
- Entrez Gene: 694915 Rhesus monkey
- Omim: 609022 Human
see all -
別名
- AVO3 antibody
- AVO3 homolog antibody
- DKFZp686B11164 antibody
see all
画像
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Western blot - Anti-RICTOR antibody (ab70374)All lanes : Anti-RICTOR antibody (ab70374) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 5 µg
Lane 2 : HeLa whole cell lysate at 2.5 µg
Lane 3 : HeLa whole cell lysate at 1 µg
Lane 4 : HeLa whole cell lysate at 0.5 µg
Lane 5 : NIH3T3 cells at 50 µg
Predicted band size: 192 kDa
Observed band size: 192 kDa
Additional bands at: 78 kDa, 80 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RICTOR antibody (ab70374)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling RICTOR with ab70374 at 1/1000 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-RICTOR antibody (ab70374)Detection of Human RICTOR by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 1/4 of IP loaded) using ab70374 at 3 µg/mg for IP (Lane 1) and at 0.04 µg/ml for subsequent WB detection. Lane 2 represents control rabbit IgG. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RICTOR antibody (ab70374)ab70374 (2µg/ml) staining RICTOR in human prostate using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of glandular epithelium.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (16)
ab70374 は 16 報の論文で使用されています。
- Lin S et al. Targeting parvalbumin promotes M2 macrophage polarization and energy expenditure in mice. Nat Commun 13:3301 (2022). PubMed: 35676256
- Chen Q et al. PEBP4 Directs the Malignant Behavior of Hepatocellular Carcinoma Cells via Regulating mTORC1 and mTORC2. Int J Mol Sci 23:N/A (2022). PubMed: 35955931
- Zhou H et al. Epithelial-mesenchymal reprogramming by KLF4-regulated Rictor expression contributes to metastasis of non-small cell lung cancer cells. Int J Biol Sci 18:4869-4883 (2022). PubMed: 35982899
- Cao RZ et al. Rictor Activates Cav 1 Through the Akt Signaling Pathway to Inhibit the Apoptosis of Gastric Cancer Cells. Front Oncol 11:641453 (2021). PubMed: 34540654
- Feng G et al. G-Protein-Coupled Estrogen Receptor 1 Promotes Gender Disparities in Hepatocellular Carcinoma via Modulation of SIN1 and mTOR Complex 2 Activity. Mol Cancer Res 18:1863-1875 (2020). PubMed: 32873626