Anti-RICTOR 抗体 (ab32892)
Key features and details
- Goat polyclonal to RICTOR
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-RICTOR antibody
RICTOR 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to RICTOR -
由来種
Goat -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Dog
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免疫原
Synthetic peptide:
(C)KQPIVDTSAES
, corresponding to C terminal amino acids 1698-1708 of Human RICTOR -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32892の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development. -
配列類似性
Belongs to the RICTOR family. -
翻訳後修飾
Phosphorylated by MTOR; when part of mTORC2. Phosphorylated at Thr-1135 by RPS6KB1; phosphorylation of RICTOR inhibits mTORC2 and AKT1 signaling. - Information by UniProt
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参照データベース
- Entrez Gene: 479356 Dog
- Entrez Gene: 253260 Human
- Entrez Gene: 78757 Mouse
- Omim: 609022 Human
- SwissProt: Q6R327 Human
- SwissProt: Q6QI06 Mouse
- Unigene: 407926 Human
- Unigene: 275811 Mouse
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別名
- AVO3 antibody
- AVO3 homolog antibody
- DKFZp686B11164 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RICTOR antibody (ab32892)Ab32892 (2.5ug/ml) staining human RICTOR in human kidney tissue by immunohistochemistry using paraffin embedded tissue.
Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
In paraffin embedded Kidney shows strong cytoplasmic staining of epithelial cells in distal convoluted tubules.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab32892 は 1 報の論文で使用されています。
- Joha S et al. GILZ inhibits the mTORC2/AKT pathway in BCR-ABL(+) cells. Oncogene : (2011). PubMed: 21804606