Anti-RICTOR 抗体 [1G3P2C9] (ab176850)
![Western blot - Anti-RICTOR antibody [1G3P2C9] (ab176850)](https://www.abcam.co.jp/ps/products/176/ab176850/Images/ab176850-204560-anti-rictor-antibody-1g3p2c9-western-blot.jpg "Western blot - Anti-RICTOR antibody [1G3P2C9] (ab176850)")
Key features and details
- Mouse monoclonal [1G3P2C9] to RICTOR
- Suitable for: ICC, IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
-
製品名
Anti-RICTOR antibody [1G3P2C9]
RICTOR 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [1G3P2C9] to RICTOR -
由来種
Mouse -
アプリケーション
適用あり: ICC, IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
-
免疫原
Synthetic peptide within Human RICTOR aa 1650-1708 (C terminal). The exact sequence is proprietary.
Sequence:I CLYSEVSHLL SHCTFRLPCR RFIQELFQDV QFLQMHEEAE AVLATPPKQP IVDTSAES
Database link: Q6R327 -
エピトープ
The epitope recognized by ab176850 maps to a region between residue 1650 and the C-terminus (residue 1708) using the numbering given in Q6R327. -
ポジティブ･コントロール
- IHC-P: Human prostate carcinoma tissue. WB: 293T whole cell lysates. ICC: HeLa cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab176850 was affinity purified using an epitope specific to RICTOR immobilized on solid support. -
ポリ/モノ
モノクローナル -
クローン名
1G3P2C9 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab176850の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
1/500 - 1/2000.
|
|
| IHC-P |
1/100 - 1/500.
|
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 192 kDa.
|
| 特記事項 |
|---|
|
ICC
1/500 - 1/2000. |
|
IHC-P
1/100 - 1/500. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 192 kDa. |
ターゲット情報
-
機能
Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development. -
配列類似性
Belongs to the RICTOR family. -
翻訳後修飾
Phosphorylated by MTOR; when part of mTORC2. Phosphorylated at Thr-1135 by RPS6KB1; phosphorylation of RICTOR inhibits mTORC2 and AKT1 signaling. - Information by UniProt
-
参照データベース
- Entrez Gene: 461907 Chimpanzee
- Entrez Gene: 479356 Dog
- Entrez Gene: 101132782 Gorilla
- Entrez Gene: 253260 Human
- Entrez Gene: 100443248 Orangutan
- Entrez Gene: 694915 Rhesus monkey
- Omim: 609022 Human
- SwissProt: Q6R327 Human
see all -
別名
- AVO3 antibody
- AVO3 homolog antibody
- DKFZp686B11164 antibody
see all
画像
-
Western blot - Anti-RICTOR antibody [1G3P2C9] (ab176850)All lanes : Anti-RICTOR antibody [1G3P2C9] (ab176850) at 1 µg/ml
Lane 1 : 293T whole cell lysates at 50 µg
Lane 2 : 293T whole cell lysates at 15 µg
Lane 3 : 293T whole cell lysates at 5 µg
Developed using the ECL technique.
Predicted band size: 192 kDa
Exposure time: 10 seconds -
![Immunocytochemistry - Anti-RICTOR antibody [1G3P2C9] (ab176850)](https://www.abcam.co.jp/ps/products/176/ab176850/Images/ab176850-467869-anti-rictor-antibody-immunocytochemistry-hela-human.jpg)
Immunocytochemistry - Anti-RICTOR antibody [1G3P2C9] (ab176850)Immunocytochemistry analysis of formaldehyde-fixed HeLa cells labeling RICTOR with ab176850 at a 1/1000 dilution.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RICTOR antibody [1G3P2C9] (ab176850)](https://www.abcam.co.jp/ps/products/176/ab176850/Images/ab176850-204559-anti-rictor-antibody-1g3p2c9-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RICTOR antibody [1G3P2C9] (ab176850)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue labeling RICTOR with ab176850 at 1/250 dilution. Detection: DAB staining.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab176850 は 1 報の論文で使用されています。
- Voisin A et al. Proteins Associated with Phagocytosis Alteration in Retinal Pigment Epithelial Cells Derived from Age-Related Macular Degeneration Patients. Antioxidants (Basel) 11:N/A (2022). PubMed: 35453399