AB217132

Anti-Ribonuclease Inhibitor/RAI 抗体

Name: Anti-Ribonuclease Inhibitor/RAI 抗体 (ab217132) | Abcam
Brand: Abcam Limited
SKU: AB217132
Price: 74000 JPY
Availability: InStock

Anti-Ribonuclease Inhibitor/RAI antibody

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(2 Publications)

Rabbit Polyclonal Ribonuclease Inhibitor/RAI antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RNH1 aa 1-50.

別名を表示する

PRI, RNH, RNH1, Ribonuclease inhibitor, Placental ribonuclease inhibitor, Ribonuclease/angiogenin inhibitor 1, Placental RNase inhibitor, RAI

2 Images

Supplier Data

Immunoprecipitation - Anti-Ribonuclease Inhibitor/RAI antibody (AB217132)

Detection of Ribonuclease Inhibitor/RAI in immunoprecipitates of 293T whole cell lysate prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab217132 at 6 μg/mg lysate for IP (Lane 1) and at 0.4 μg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-Ribonuclease Inhibitor/RAI antibody (ab217132)

Predicted band size: 50 kDa

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Supplier Data

Western blot - Anti-Ribonuclease Inhibitor/RAI antibody (AB217132)

Lysates were prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-Ribonuclease Inhibitor/RAI antibody (ab217132) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 50 kDa

true

Exposure time: 10s

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

IP, WB

applications

免疫原

Synthetic Peptide within Human RNH1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P13489

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

ab217132 was affinity purified using an epitope specific to Ribonuclease Inhibitor/RAI immobilized on solid support.

バッファー組成

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Ribonuclease Inhibitor (RI) also known as RAI is a protein weighing approximately 50 kDa and is responsible for binding to ribonucleases (RNases) to inhibit their activity. This protein is expressed in a variety of tissues but appears most abundantly in the cytoplasm of mammalian cells. RI is characterized by its leucine-rich repeat (LRR) structure which facilitates its high-affinity binding to target RNases effectively serving to regulate ribonuclease activity within cells.

Biological function summary

Ribonuclease Inhibitor participates in protecting cellular RNA from degradation by forming stable complexes with RNases. This interaction is important for maintaining RNA stability particularly within cellular environments where mRNA turnover must be tightly controlled. RI does not only inhibit free RNases but also regulates those RNases that are secreted or released subsequently. It does not have enzymatic activity itself serving more as a guard that ensures RNA integrity for processes such as transcription translation and overall gene expression.

Pathways

The function of Ribonuclease Inhibitor fits strategically into RNA metabolism and the regulation of its turnover under various physiological conditions. RI acts within the mRNA biogenesis and degradation pathways coordinating with other proteins involved in RNA processing such as Dicer. RI's role is essential in fine-tuning RNA levels and preventing unwanted cellulalr RNA cleavage which can be directed by pathways involving diverse sets of RNases across different cellular contexts.

Researchers have observed connections between Ribonuclease Inhibitor and conditions such as cancer and neurodegenerative diseases. For instance disrupting RI balance can result in excessive RNA degradation contributing to pathologies associated with abnormal mRNA destruction in cells. It often aligns in regulatory functions with proteins like angiogenin where disrupted RI-angiogenin interactions have been implicated in tumor growth and progression. Additionally RI's dysfunction is associated with neuroinflammatory conditions where RNA stability plays critical roles.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and angiogenin (ANG) (PubMed : 12578357, PubMed : 14515218, PubMed : 3219362, PubMed : 3243277, PubMed : 3470787, PubMed : 9050852). May play a role in redox homeostasis (PubMed : 17292889). Required to inhibit the cytotoxic tRNA ribonuclease activity of ANG in the cytoplasm in absence of stress (PubMed : 23843625, PubMed : 32510170). Relocates to the nucleus in response to stress, relieving inhibition of ANG in the cytoplasm, and inhibiting the angiogenic activity of ANG in the nucleus (PubMed : 23843625).

See full target information RNH1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceutics 14: PubMed35745743

2022

Bovine Pancreatic RNase A: An Insight into the Mechanism of Antitumor Activity In Vitro and In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Islam Saber Ead Mohamed,Aleksandra V Sen'kova,Oleg V Markov,Andrey V Markov,Innokenty A Savin,Marina A Zenkova,Nadezhda L Mironova

Virulence 11:607-635 PubMed32420802

2020

TMT-based quantitative proteomics analysis reveals the attenuated replication mechanism of Newcastle disease virus caused by nuclear localization signal mutation in viral matrix protein.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqiang Duan,Chao Yuan,Yifan Han,Lei Zhou,Jiafu Zhao,Yong Ruan,Jiaqi Chen,Mengmeng Ni,Xinqin Ji

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

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For licensing inquiries, please contact partnerships@abcam.com

Anti-Ribonuclease Inhibitor/RAI 抗体

Anti-Ribonuclease Inhibitor/RAI antibody

Immunoprecipitation - Anti-Ribonuclease Inhibitor/RAI antibody (AB217132)

Western blot - Anti-Ribonuclease Inhibitor/RAI antibody (AB217132)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise