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AB83943

Anti-Ribonuclease H2, subunit A/RNASEH2A 抗体

Anti-Ribonuclease H2, subunit A/RNASEH2A antibody

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(7 Publications)

Rabbit Polyclonal Ribonuclease H2, subunit A/RNASEH2A antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 7 publications.

別名を表示する

RNASEHI, RNHIA, RNASEH2A, Ribonuclease H2 subunit A, RNase H2 subunit A, Aicardi-Goutieres syndrome 4 protein, RNase H(35), Ribonuclease HI large subunit, Ribonuclease HI subunit A, AGS4, RNase HI large subunit

3 Images
Immunocytochemistry - Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (AB83943)
  • ICC

Unknown

Immunocytochemistry - Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (AB83943)

Immunocytochemistry analysis of HeLa cells using ab83943 at 2μg/ml.

Immunocytochemistry - Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (AB83943)
  • ICC

Unknown

Immunocytochemistry - Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (AB83943)

Immunofluorescence of Rnse H2A in Hela cells using ab83943 at 4.75 ug/ml.

Western blot - Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (AB83943)
  • WB

Unknown

Western blot - Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (AB83943)

All lanes:

Western blot - Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (ab83943) at 1 µg/mL

All lanes:

HeLa cell lysate at 15 µg

Predicted band size: 33 kDa

Observed band size: 33 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

ICC, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "2-20 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Ribonuclease H2 subunit A also known as RNASEH2A is one part of the RNase H2 enzyme complex. This protein weighs approximately 33 kilodaltons and plays an important role in RNA-DNA hybrid processing. RNASEH2A operates by cleaving the RNA strand of RNA-DNA hybrids which is necessary for maintaining genome stability. The protein expresses in a variety of tissues. Importantly it is found in high levels in cells with rapid division like embryonic and stem cells.
Biological function summary

RNASEH2A facilitates the removal of ribonucleotides from DNA which helps in DNA replication and repair. As a part of the RNase H2 complex alongside subunits B and C it ensures the correct removal of embedded ribonucleotides from DNA strands. This process is important for maintaining the integrity of the genome and preventing mutations. The activity of RNASEH2A directly impacts DNA replication fidelity and overall genome stability.

Pathways

RNASEH2A plays an important role in the maintenance of genome integrity and the DNA repair pathway. It directly interacts with the process of ribonucleotide excision repair which prevents genomic instability. RNASEH2A works alongside enzymes involved in DNA replication and repair mechanisms like DNA polymerases to correct DNA strands. The collaboration of these proteins ensures the accuracy of genomic information passed during cell division.

RNASEH2A is associated with Aicardi-Goutières syndrome a severe neurodevelopmental disorder. This syndrome results from mutations in any of the RNase H2 subunits including RNASEH2A leading to accumulated DNA damage and a chronic interferon response. Another condition linked to malfunctions in this protein is systemic lupus erythematosus where an abnormal immune response attacks the body’s own cells. Both conditions connect RNASEH2A with the regulation of immune responses and highlight its role in preventing inappropriate autoimmunity.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA : DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA : RNA duplexes.
See full target information RNASEH2A
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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 52:11641-11658 PubMed39189461

2024

Synergistic action of human RNaseH2 and the RNA helicase-nuclease DDX3X in processing R-loops.

Applications

Unspecified application

Species

Unspecified reactive species

Massimiliano Secchi,Anna Garbelli,Valentina Riva,Graziano Deidda,Carolina Santonicola,Teresa Maria Formica,Simone Sabbioneda,Emmanuele Crespan,Giovanni Maga

Nature communications 15:1943 PubMed38431617

2024

RTF2 controls replication repriming and ribonucleotide excision at the replisome.

Applications

Unspecified application

Species

Unspecified reactive species

Brooke A Conti,Penelope D Ruiz,Cayla Broton,Nicolas J Blobel,Molly C Kottemann,Sunandini Sridhar,Francis P Lach,Tom F Wiley,Nanda K Sasi,Thomas Carroll,Agata Smogorzewska

Heliyon 9:e18127 PubMed37520960

2023

SP1/RNASEH2A accelerates the development of hepatocellular carcinoma by regulating EMT.

Applications

Unspecified application

Species

Unspecified reactive species

Yunhe Hao,Rui Zou,Jiashou Tao,Manfei Jiang,Duo Li

Environmental toxicology 36:2093-2104 PubMed34254728

2021

Long noncoding RNA LINC01287 promotes proliferation and inhibits apoptosis of lung adenocarcinoma cells via the miR-3529-5p/RNASEH2A axis under the competitive endogenous RNA pattern.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhang,Dan Ma,Honggang Kang,Jun Zhao,Mengxiang Yang

Nucleic acids research 48:11551-11565 PubMed33137198

2020

Novel alternative ribonucleotide excision repair pathways in human cells by DDX3X and specialized DNA polymerases.

Applications

Unspecified application

Species

Unspecified reactive species

Valentina Riva,Anna Garbelli,Federica Casiraghi,Francesca Arena,Claudia Immacolata Trivisani,Assunta Gagliardi,Luca Bini,Martina Schroeder,Antonio Maffia,Simone Sabbioneda,Giovanni Maga

Molecular cell 76:600-616.e6 PubMed31679819

2019

R-Loops Promote Antisense Transcription across the Mammalian Genome.

Applications

Unspecified application

Species

Unspecified reactive species

Sue Mei Tan-Wong,Somdutta Dhir,Nick J Proudfoot

PloS one 8:e76084 PubMed24086692

2013

The voltage-gated sodium channel nav1.8 is expressed in human sperm.

Applications

Unspecified application

Species

Unspecified reactive species

Antonio Cejudo-Roman,Francisco M Pinto,Nerea Subirán,Cristina G Ravina,Manuel Fernández-Sánchez,Natalia Pérez-Hernández,Ricardo Pérez,Alberto Pacheco,Jon Irazusta,Luz Candenas
View all publications
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代替製品

Primary Antibodies

AB92876

Anti-Ribonuclease H2, subunit A/RNASEH2A antibody

Immunocytochemistry/ Immunofluorescence - Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (AB92876)

  • 0
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