Anti-RhoGAP 抗体 [EP489Y] (ab32328)
![Flow Cytometry (Intracellular) - Anti-RhoGAP antibody [EP489Y] (ab32328)](https://www.abcam.co.jp/ps/products/32/ab32328/Images/ab32328-238343-anti-rhogap-antibody-ep489y-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Anti-RhoGAP antibody [EP489Y] (ab32328)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP489Y] to RhoGAP
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-RhoGAP antibody [EP489Y]
RhoGAP 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP489Y] to RhoGAP -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, Flow Cyt (Intra)more details
適用なし: ICC/IF or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human RhoGAP aa 1150-1250. The exact sequence is proprietary.
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ポジティブ･コントロール
- Human stomach carcinoma and the following cell lysates: Hela, NIH 3T3, Jurkat, 293, K562, MCF-7, A431 and PC12.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP489Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32328の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500. Detects a band of approximately 190 kDa (predicted molecular weight: 171 kDa).
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| IHC-P | (2) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Flow Cyt (Intra) |
1/60 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
WB
1/500. Detects a band of approximately 190 kDa (predicted molecular weight: 171 kDa). |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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Flow Cyt (Intra)
1/60 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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関連性
The p190 RhoGAP protein is associated with p120 RasGAP in growth-factor stimulated and tyrosine kinase transformed cells. It functions as a GTPase - activating protein (GAP) for Rho and Rac family proteins, which are involved in regulating cytoskeletal actin and membrane ruffling. The antibody appears to couple signal transduction via Ras and Rho through its association with RasGAP. The presence of two adjacent SH2 domains in the p21 RAS GAP indicates that GAP might interact directly with tyrosine kinases. p190B can be directly phosphorylated on a single identified tyrosine residue by the activated insulin and IGF-1 receptors. This phosphorylation causes p190B to translocate to the lipid rafts of the plasma membrane, where it can facilitate the inactivation of the Rho GTPase. P190B also has been shown to be tyrosine phosphorylated by cSrc and vSrc. Additionally the GAP domain of p190B is shown to attenuate signal transducing activity of Rac, Rho and CDC42. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 394 Human
- Entrez Gene: 11855 Mouse
- Entrez Gene: 299012 Rat
- Omim: 602680 Human
- SwissProt: Q13017 Human
- SwissProt: P97393 Mouse
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別名
- ARHGAP 5 antibody
- ARHGAP5 antibody
- p190 B antibody
see all
画像
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Flow Cytometry (Intracellular) - Anti-RhoGAP antibody [EP489Y] (ab32328)
Overlay histogram showing A431 cells stained with ab32328 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32328, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Western blot - Anti-RhoGAP antibody [EP489Y] (ab32328)](https://www.abcam.co.jp/ps/products/32/ab32328/Images/ab32328-213891-anti-rhogap-antibody-ep489y-western-blot.jpg)
Western blot - Anti-RhoGAP antibody [EP489Y] (ab32328)This image is courtesy of an anonymous AbreviewAnti-RhoGAP antibody [EP489Y] (ab32328) at 1/500 dilution + Mouse MEFs whole cell lysate at 50 µg
Secondary
HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 171 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
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![Western blot - Anti-RhoGAP antibody [EP489Y] (ab32328)](https://www.abcam.co.jp/ps/products/32/ab32328/Images/ab32328-44470-anti-rhogap-antibody-ep489y-western-blot.jpg)
Western blot - Anti-RhoGAP antibody [EP489Y] (ab32328)All lanes : Anti-RhoGAP antibody [EP489Y] (ab32328) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MCF-7 cell lysate
Lane 4 : K562 cell lysate
Lane 5 : A431 cell lysate
Lane 6 : 293 cell lysate
Lane 7 : NIH 3T3 cell lysate
Lane 8 : PC12 cell lysate
Predicted band size: 171 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoGAP antibody [EP489Y] (ab32328)](https://www.abcam.co.jp/ps/products/32/ab32328/Images/ab32328-44469-anti-rhogap-antibody-ep489y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoGAP antibody [EP489Y] (ab32328)ab32328, at a 1/100 dilution, staining human RhoGAP in stomach carcinoma by Immunohistochemistry, Paraffin embedded tissue
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-RhoGAP antibody [EP489Y] (ab32328)](https://www.abcam.co.jp/ps/products/32/ab32328/Images/ab32328-5-benefits-of-recombinant-antibodies.png)
Anti-RhoGAP antibody [EP489Y] (ab32328)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab32328 は 4 報の論文で使用されています。
- Yang C et al. Transcriptomic Analysis Identified ARHGAP Family as a Novel Biomarker Associated With Tumor-Promoting Immune Infiltration and Nanomechanical Characteristics in Bladder Cancer. Front Cell Dev Biol 9:657219 (2021). PubMed: 34307347
- Kim SR et al. Clinical Significance of CLDN18.2 Expression in Metastatic Diffuse-Type Gastric Cancer. J Gastric Cancer 20:408-420 (2020). PubMed: 33425442
- Zhu L et al. Impaired autophagic degradation of lncRNA ARHGAP5-AS1 promotes chemoresistance in gastric cancer. Cell Death Dis 10:383 (2019). PubMed: 31097692
- Repetto D et al. p140Cap regulates memory and synaptic plasticity through Src-mediated and citron-N-mediated actin reorganization. J Neurosci 34:1542-53 (2014). Mouse . PubMed: 24453341