Anti-Rhodopsin 抗体 [1D4] (ab5417)
Key features and details
- Mouse monoclonal [1D4] to Rhodopsin
- Suitable for: ELISA, IHC-FoFr, IP, WB, IHC-Fr, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Cow, Human, Zebrafish, Amphibian
- Isotype: IgG1
製品の概要
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製品名
Anti-Rhodopsin antibody [1D4]
Rhodopsin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [1D4] to Rhodopsin -
由来種
Mouse -
特異性
ab5417 detects Rhodopsin from human and bovine retinal samples. Data from Yin J et al., 2012 (PMID 22743318) indicates that in Zebrafish ab5417 appears to recognize Red Opsin rather than Rhodopsin.
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アプリケーション
適用あり: ELISA, IHC-FoFr, IP, WB, IHC-Fr, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Cow, Human, Zebrafish, Amphibian
交差が予測される動物種: Rabbit -
免疫原
Tissue, cells or virus corresponding to Bovine Rhodopsin. Bleached bovine ROS [rod outer segment] disk membranes
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エピトープ
The epitope for this antibody has been localized to the C-terminal nine amino acids of bovine rhodopsin known as the 1D4 epitope. -
ポジティブ・コントロール
- WB: HL60 whole cell lysate IHC-P: Human and mouse retinal tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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精製度
Protein G purified -
一次抗体 備考
Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light to an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin’s activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin. -
ポリ/モノ
モノクローナル -
クローン名
1D4 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5417の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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IHC-FoFr |
Use at an assay dependent concentration. PubMed: 19587120
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IP |
Use at an assay dependent concentration.
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WB | (2) |
1/100 - 1/1000. Detects a band of approximately 40 kDa.
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IHC-Fr | (1) |
Use at an assay dependent concentration. PubMed: 22743318
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IHC-P |
1/100 - 1/1000.
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ICC/IF | (1) |
Use at an assay dependent concentration.
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
IHC-FoFr
Use at an assay dependent concentration. PubMed: 19587120 |
IP
Use at an assay dependent concentration. |
WB
1/100 - 1/1000. Detects a band of approximately 40 kDa. |
IHC-Fr
Use at an assay dependent concentration. PubMed: 22743318 |
IHC-P
1/100 - 1/1000. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal. -
組織特異性
Rod shaped photoreceptor cells which mediates vision in dim light. -
関連疾患
Retinitis pigmentosa 4
Night blindness, congenital stationary, autosomal dominant 1 -
配列類似性
Belongs to the G-protein coupled receptor 1 family. Opsin subfamily. -
翻訳後修飾
Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
Contains one covalently linked retinal chromophore. -
細胞内局在
Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia. - Information by UniProt
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参照データベース
- Entrez Gene: 509933 Cow
- Entrez Gene: 100307115 Human
- Entrez Gene: 6010 Human
- Entrez Gene: 212541 Mouse
- Entrez Gene: 100009200 Rabbit
- Entrez Gene: 24717 Rat
- Entrez Gene: 30295 Zebrafish
- Omim: 180380 Human
see all -
別名
- CSNBAD1 antibody
- MGC138309 antibody
- MGC138311 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)Pearring et al PLoS Genet. 2017 Apr 14;13(4):e1006740. doi: 10.1371/journal.pgen.1006740. eCollection 2017 Apr. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Deletion of Arf4 mice from the retina does not disrupt rhodopsin localization or photoreceptor morphology.
E. Arf4 immunostaining in Arf4flox/CagCreER experimental and control retinal cross-sections. Image of the photoreceptor IS where the biosynthetic membranes are localized. Eyes were collected at P34. Scale bar = 10 μm.
F. Rhodopsin immunostaining in Arf4flox/CagCreER experimental and control retinal cross-sections. Eyes were collected at P34. Scale bar = 10 μm.
G. Comparative analysis of photoreceptor morphology in Arf4flox/CagCreER experimental and control retinal cross-sections. Eyes were collected at P41. Scale bar = 20 μm.
OS = outer segment, IS = inner segment, ONL = outer nuclear layer, OPL = outer plexiform layer.
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Anti-Rhodopsin antibody [1D4] (ab5417) at 1/500 dilution + HL60 (Human promyelocytic leukemia cell line) cell lysate at 25 µg
Observed band size: 40 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
Immunohistochemical analysis of formalin-fixed mouse retinal tissue, labeling rhodopsin with ab5417 at a 1:50 dilution in 3% BSA-PBS solution and incubated at 4°C overnight in a high humidity environment.
A DyLight® 488 secondary antibody was used (green) incubated at room temperature in the dark. The tissue was counterstained with DAPI against DNA, showing nuclear compartments. Prior to staining the formalin-fixed tissue was permeabilized with 0.1% Triton X-100 in TBS for between 5 and 10 minutes, then blocked with 3% BSA-PBS for 30 minutes at room temperature.
The left image is a negative control with only the secondary antibody and the right image is in the presence of ab5417 and the secondary.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
Immunohistochemical analysis of formalin-fixed human retinal tissue, labeling rhodopsin with ab5417 at a 1:50 dilution in 3% BSA-PBS solution and incubated at 4°C overnight in a high humidity environment.
A DyLight® 488 secondary antibody was used (green) incubated at room temperature in the dark. The tissue was counterstained with DAPI against DNA, showing nuclear compartments. Prior to staining the formalin-fixed tissue was permeabilized with 0.1% Triton X-100 in TBS for between 5 and 10 minutes, then blocked with 3% BSA-PBS for 30 minutes at room temperature.
The left image is a negative control with only the secondary antibody and the right image is in the presence of ab5417 and the secondary.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
Immunohistochemical analysis of paraffin-embedded mouse retinal tissue labeling Rhodopsin with ab5417.
Secondary used was HRP conjugated. Prior preparation was initiated by antigen retrival using 10mM sodium citrate at pH 6.0, then the sample was microwaved for 8 to 15 minutes. Subsequent to retrival the retinal tissue was blocked for 15 minutes at room temperature with 3% hydrogen peroxide. The sample was then incubated with ab5417 in 3% BSA-PBS at 4°C at a dilution of 1:1000, overnight. Hematoxylin was used to counterstain the tissue.
The left side of the image is shown as a negative control and is the tissue in the absence of ab5417, the right side is in the prescence of the counterstain, ab5417 and the HRP conjugated secondary.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
Immunohistochemical analysis of paraffin-embedded human retinal tissue labeling Rhodopsin with ab5417.
Secondary used was HRP conjugated. Prior preparation was initiated by antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Subsequent to retrival the retinal tissue was blocked in 3% H2O2-methanol for 15 min at room temperature. The sample was then incubated with ab5417 in 3% BSA-PBS at a dilution of 1:200 overnight at 4°C, overnight. Hematoxylin was used to counterstain the tissue.
The left side of the image is shown as a negative control and is the tissue in the absence of ab5417, the right side is in the prescence of the counterstain, ab5417 and the HRP conjugated secondary.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (108)
ab5417 は 108 報の論文で使用されています。
- Dias SB et al. Age-Related Changes of the Synucleins Profile in the Mouse Retina. Biomolecules 13:N/A (2023). PubMed: 36671565
- Angueyra JM et al. Transcription factors underlying photoreceptor diversity. Elife 12:N/A (2023). PubMed: 36745553
- Zhang X et al. Transcriptome Analysis of Retinal and Choroidal Pathologies in Aged BALB/c Mice Following Systemic Neonatal Murine Cytomegalovirus Infection. Int J Mol Sci 24:N/A (2023). PubMed: 36901754
- Yang S et al. Loss of flrt2 gene leads to microphthalmia in zebrafish. Biol Open 12:N/A (2023). PubMed: 37259881
- Liu X et al. Allele-specific gene-editing approach for vision loss restoration in RHO-associated retinitis pigmentosa. Elife 12:N/A (2023). PubMed: 37272616