Anti-Rhodamine 抗体 [11H10] (ab9093)
Key features and details
- Mouse monoclonal [11H10] to Rhodamine
- Suitable for: Dot blot, ELISA
- Reacts with: Species independent
- Isotype: IgG1
製品の概要
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製品名
Anti-Rhodamine antibody [11H10] -
製品の詳細
Mouse monoclonal [11H10] to Rhodamine -
由来種
Mouse -
特異性
Rhodamine isomer 5 and isomer 6 are reactive as TAMRA, as well as TRITC conjugated proteins. No reaction is observed against Texas Red.
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アプリケーション
適用あり: Dot blot, ELISAmore details -
種交差性
交差種: Species independent -
免疫原
Chemical/ Small Molecule corresponding to Rhodamine conjugated to keyhole limpet haemocyanin.
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特記事項
The molecular formula of 5-carboxytetramethylrhodamine, succinimidyl ester (5-TAMRA, SE)is C29H25N3O7 The molecular weight of 5-TAMRA, SE is 527.53 This product contains 0.01% (w/v) Sodium Azide preservative.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
RHODAMINE Monoclonal Antibody was protein A purified and reacts specifically with Rhodamine and its derivatives. -
ポリ/モノ
モノクローナル -
クローン名
11H10 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9093の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Dot blot |
1/2000.
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ELISA |
1/500 - 1/1000.
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特記事項 |
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Dot blot
1/2000. |
ELISA
1/500 - 1/1000. |
画像
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A dilution series of two TAMRA labeled peptides were bound to a PVDF membrane using a dot blot apparatus. Peptide #1 is conjugated directly to TAMRA via an amide bond whereas peptide #2 contains an aminohexanoyl linker between the TAMRA and the peptide. Also note that the peptides differ in sequence and in length, peptide #1 has 15 amino acids and peptide #2 has 29 amino acids. A dilution series for each peptide was made starting with approximately 0.5 nmoles of peptide and followed by a series of 1:2 dilutions. After binding of the peptides to the PVDF membrane, the membrane was then blocked in 5% milk in 1X TBST for 1 hour at room temperature. The membrane was then incubated in with the ab9093 antibody at a 1:2000 dilution in 5% milk in 1X TBST for 1 hour at room temperature. Following incubation with the primary antibody the blot was then washed 3 times in 1X TBST and then incubated with a 1:10,000 dilution of an anti-mouse-HRP secondary antibody for 45 minutes at room temperature
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (5)
ab9093 は 5 報の論文で使用されています。
- Butler E et al. Exon skipping in genes encoding lineage-defining myogenic transcription factors in rhabdomyosarcoma. Cold Spring Harb Mol Case Stud 8:N/A (2022). PubMed: 35933111
- Mujagic A et al. Antioxidant nanomedicine with cytoplasmic distribution in neuronal cells shows superior neurovascular protection properties. Brain Res 1743:146922 (2020). PubMed: 32504549
- Redd MA et al. Patterned human microvascular grafts enable rapid vascularization and increase perfusion in infarcted rat hearts. Nat Commun 10:584 (2019). PubMed: 30718840
- Hosoo H et al. Neurovascular Unit Protection From Cerebral Ischemia-Reperfusion Injury by Radical-Containing Nanoparticles in Mice. Stroke 48:2238-2247 (2017). PubMed: 28655813
- Opsahl ML & Kennedy PG Early and late HHV-6 gene transcripts in multiple sclerosis lesions and normal appearing white matter. Brain 128:516-27 (2005). PubMed: 15659422