Anti-RHOB 抗体 [2916C2a] (ab53743)
![Western blot - Anti-RHOB antibody [2916C2a] (ab53743)](https://www.abcam.co.jp/ps/products/53/ab53743/Images/ab53743-66592-anti-rhob-antibody-2916c2a-western-blot.jpg "Western blot - Anti-RHOB antibody [2916C2a] (ab53743)")
Key features and details
- Mouse monoclonal [2916C2a] to RHOB
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
製品の概要
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製品名
Anti-RHOB antibody [2916C2a]
RHOB 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2916C2a] to RHOB -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Recombinant fragment -
免疫原
Recombinant fragment (Human)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purified from culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
2916C2a -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53743の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
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| 特記事項 |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa. |
ターゲット情報
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機能
Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. -
配列類似性
Belongs to the small GTPase superfamily. Rho family. -
翻訳後修飾
Prenylation specifies the subcellular location of RHOB. The farnesylated form is localized to the plasma membrane while the geranylgeranylated form is localized to the endosome. -
細胞内局在
Late endosome membrane. Cell membrane. Nucleus. Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus. - Information by UniProt
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別名
- Aplysia RAS-related homolog 6 antibody
- ARH6 antibody
- ARHB antibody
see all
画像
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Western blot - Anti-RHOB antibody [2916C2a] (ab53743)Anti-RHOB antibody [2916C2a] (ab53743) + immunized recombinant protein
Predicted band size: 22 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
The band on the Wetstern blot does not correspond to the predicted size, as the recombinant protein was used as the test antigen
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab53743 は 4 報の論文で使用されています。
- Yao X et al. LncRNA GAS5 Regulates Osteosarcoma Cell Proliferation, Migration, and Invasion by Regulating RHOB via Sponging miR-663a. Cancer Manag Res 12:8253-8261 (2020). PubMed: 32982421
- Liu H et al. Exploration of vascular adhesion protein-1 expression in patients with conjunctivitis associated systemic lupus erythematosus using 2D-DIGE. Exp Ther Med 18:5072-5077 (2019). PubMed: 31819770
- Quinn K et al. Rho GTPases modulate entry of Ebola virus and vesicular stomatitis virus pseudotyped vectors. J Virol 83:10176-86 (2009). PubMed: 19625394
- Murray D et al. NET1-mediated RhoA activation facilitates lysophosphatidic acid-induced cell migration and invasion in gastric cancer. Br J Cancer 99:1322-9 (2008). WB ; Human . PubMed: 18827818