Anti-RhoA (phospho S188) 抗体 (ab41435)
Key features and details
- Rabbit polyclonal to RhoA (phospho S188)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RhoA (phospho S188) antibody
RhoA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RhoA (phospho S188) -
由来種
Rabbit -
特異性
Phospho-Ser188-RhoA specific. RhoA immunogen sequence not found in RhoB or RhoC proteins. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide corresponding to Human RhoA (phospho S188) conjugated to keyhole limpet haemocyanin.
Database link: P06749 -
特記事項
Do not aliquot.Post-translational regulation of Rho activity has been shown specifically for RhoA. This Rho protein is phosphorylated in vitro on serine 188 by cAMP- and cGMP-dependent kinases (PKA and PKG). Ser188 phosphorylation enhances RhoGDI binding and inhibits RhoA-mediated stress fiber formation. Thus, Ser188 is an important site for negative regulation of RhoA activity.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
一次抗体 備考
Post-translational regulation of Rho activity has been shown specifically for RhoA. This Rho protein is phosphorylated in vitro on serine 188 by cAMP- and cGMP-dependent kinases (PKA and PKG). Ser188 phosphorylation enhances RhoGDI binding and inhibits RhoA-mediated stress fiber formation. Thus, Ser188 is an important site for negative regulation of RhoA activity. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab41435の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (9) |
1/1000. Predicted molecular weight: 22 kDa.
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| 特記事項 |
|---|
|
WB
1/1000. Predicted molecular weight: 22 kDa. |
ターゲット情報
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機能
Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. -
配列類似性
Belongs to the small GTPase superfamily. Rho family. -
ドメイン
The basic-rich region is essential for yopT recognition and cleavage. -
翻訳後修飾
Substrate for botulinum ADP-ribosyltransferase.
Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.
AMPylation at Tyr-34 and Thr-37 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
Ubiquitinated by the BCR(BACURD1) and BCR(BACURD2) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. -
細胞内局在
Cell membrane. Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 387 Human
- Entrez Gene: 117273 Rat
- Omim: 165390 Human
- SwissProt: P61586 Human
- SwissProt: P61589 Rat
- Unigene: 247077 Human
- Unigene: 107401 Rat
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別名
- Aplysia ras related homolog 12 antibody
- ARH12 antibody
- ARHA antibody
see all
画像
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Western blot - Anti-RhoA (phospho S188) antibody (ab41435)Lanes 1-2 : anti-Rho
Lanes 3-6 : Anti-RhoA (phospho S188) antibody (ab41435) at 1/1000 dilution
Lane 1 : recombinant human RhoA GST fusion protein, unphosphorylated
Lane 2 : recombinant human RhoA GST fusion protein, phosphorylated with PKA
Lane 3 : recombinant human RhoA GST fusion protein, unphosphorylated
Lane 4 : recombinant human RhoA GST fusion protein, phosphorylated with PKA
Lane 5 : recombinant human RhoA GST fusion protein, phosphorylated with PKA
with RhoA (phospho S188) peptide
Lane 6 : recombinant human RhoA GST fusion protein, phosphorylated with PKA with non-specific phosphoserine peptide
Predicted band size: 22 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.A fusion protein was used in this western blot; hence the difference in molecular weight compared to predicted molecular weight.
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Western blot - Anti-RhoA (phospho S188) antibody (ab41435)This image is courtesy of an anonymous abreview.All lanes : Anti-RhoA (phospho S188) antibody (ab41435) at 1/1000 dilution
Lane 1 : MCF7
Lane 2 : human liver nuclear tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Polyclonal goat anti-rabbit HRP conjugate at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 25 secondsBlocking: 5% milk for 1 hour at 23°C
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (30)
ab41435 は 30 報の論文で使用されています。
- Zhang X et al. Soluble TREM2 ameliorates tau phosphorylation and cognitive deficits through activating transgelin-2 in Alzheimer's disease. Nat Commun 14:6670 (2023). PubMed: 37865646
- Jia W et al. Negative regulation of HBG1/2 expression through S6K by long noncoding RNA NR_120526. Transl Pediatr 12:907-917 (2023). PubMed: 37305725
- Liu X et al. Pre-miRNA Hsa-Let-7a-2: a Novel Intracellular Partner of Angiotensin II Type 2 Receptor Negatively Regulating its Signals. Int J Biol Sci 18:3237-3250 (2022). PubMed: 35637969
- Yan L et al. Mex-3 RNA binding MEX3A promotes the proliferation and migration of breast cancer cells via regulating RhoA/ROCK1/LIMK1 signaling pathway. Bioengineered 12:5850-5858 (2021). PubMed: 34486491
- Senoo H et al. KARATE: PKA-induced KRAS4B-RHOA-mTORC2 supercomplex phosphorylates AKT in insulin signaling and glucose homeostasis. Mol Cell 81:4622-4634.e8 (2021). PubMed: 34551282