Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-RhoA + RhoC 抗体 [EPR18133] (ab187026)

Submit a review Submit a question References (3)
Western blot - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)
  • Western blot - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)
  • Western blot - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)
  • Western blot - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)
  • Immunocytochemistry/ Immunofluorescence - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)
  • Flow Cytometry (Intracellular) - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)
  • Immunoprecipitation - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)
  • Anti-RhoA + RhoC antibody [EPR18133] (ab187026)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18133] to RhoA + RhoC
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free PE

製品の概要

  • 製品名

    Anti-RhoA + RhoC antibody [EPR18133]
    RhoA + RhoC 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR18133] to RhoA + RhoC

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human RhoA full length protein, Human RhoC full length protein. HepG2, HeLa, Jurkat and C6, Raw264.7, PC12 and NIH 3T3 cell lysates. Human fetal brain and fetal kidney lysates; Mouse brain and kidney lysates; Rat brain and kidney lysates. IHC-P: Transitional cell carcinoma of bladder tissue, Mouse kidney and rat stomach tissues. ICC/IF: NIH3T3 cells. Flow Cyt (intra): Jurkat cells. IP: NIH/3T3 cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR18133

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab187026の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/400.
WB
1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/500.
IP
1/50.
特記事項
Flow Cyt (Intra)
1/400.
WB
1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/500.
IP
1/50.

ターゲット情報

  • 関連性

    Transforming protein RhoA: Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity). Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation; promotes KCNA2 endocytosis. Rho-related GTP-binding protein RhoC: Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Regulates apical junction formation in bronchial epithelial cells.

  • 参照データベース

    • 別名

      • ARH12 antibody
      • ARH9 antibody
      • ARHA antibody
      • ARHC antibody
      • H9 antibody
      • Rho cDNA clone 12 antibody
      • Rho cDNA clone 9 antibody
      • Rho-related GTP-binding protein RhoC antibody
      • RHO12 antibody
      • RHOA antibody
      • RHOC antibody
      • RHOH12 antibody
      • RHOH9 antibody
      • Transforming protein RhoA antibody
      see all

    画像

    • Western blot - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)
      Western blot - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)
      Lanes 1-2 : Anti-RhoA + RhoC antibody [EPR18133] (ab187026) at 1/20000 dilution
      Lane 3 : Anti-RhoA + RhoC antibody [EPR18133] (ab187026) at 1/500 dilution

      Lane 1 : Human RhoA full length protein
      Lane 2 : Human RhoB full length protein
      Lane 3 : Human RhoC full length protein

      Lysates/proteins at 0.01 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 22 kDa
      Observed band size: 24 kDa why is the actual band size different from the predicted?



      5% NFDM/TBST: Blocking and diluting buffer.

      Exposure time: Lane 1: 30 seconds; lane 2 and 3: 3 minutes.

      Human RhoA full length protein (ab101594) contains aa1-193 with His-Tag® at N-Terminus; Human RhoB full length protein (ab107139) contains aa1-193 with His-Tag® at N-Terminus; Human RhoC full length protein (ab98085) contains aa1-190 with His-Tag® at N-Terminus.

      The affinity of ab187016 to RhoA is higher than to RhoC.

    • Western blot - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)
      Western blot - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)
      All lanes : Anti-RhoA + RhoC antibody [EPR18133] (ab187026) at 1/2000 dilution

      Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
      Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
      Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 22 kDa
      Observed band size: 22 kDa


      Exposure time: 1 minute


      5% NFDM/TBST: Blocking and diluting buffer.

    • Western blot - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)
      Western blot - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)
      All lanes : Anti-RhoA + RhoC antibody [EPR18133] (ab187026) at 1/2000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Human fetal kidney lysate

      Lysates/proteins at 10 mg/ml per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 22 kDa
      Observed band size: 22 kDa


      Exposure time: 3 minutes


      5% NFDM/TBST: Blocking and diluting buffer.

    • Western blot - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)
      Western blot - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)
      All lanes : Anti-RhoA + RhoC antibody [EPR18133] (ab187026) at 1/2000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Mouse kidney lysate
      Lane 3 : Rat brain lysate
      Lane 4 : Rat kidney lysate
      Lane 5 : C6 (Rat glial tumor cells) whole cell lysate
      Lane 6 : Raw264.7(Mouse macrophage cells transformed with Abelson murine leukemia virus ) whole cell lysate
      Lane 7 : PC12 (Rat adrenal gland pheochromocytoma ) whole cell lysate
      Lane 8 : NIH 3T3 (Mouse embyro fibroblast cells ) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 22 kDa
      Observed band size: 22 kDa


      Exposure time: 10 seconds


      5% NFDM/TBST: Blocking and diluting buffer.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)

      Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling RhoA + RhoC with ab187026 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on cancer cells of transitional cell carcinoma of bladder is observed. Counter stained with Hematoxylin.

      Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)

      Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling RhoA + RhoC with ab187026 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on kidney tubules of mouse kidney is observed. Counter stained with Hematoxylin.

      Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)

      Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling RhoA + RhoC with ab187026 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on epithelial cells of rat stomach is observed. Counter stained with Hematoxylin.

      Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)
      Immunocytochemistry/ Immunofluorescence - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embyro fibroblast cells) cells labeling RhoA + RhoC with ab187026 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on NIH 3T3 cells is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
      The negative controls are as follows:
      1. ab187026 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

    • Flow Cytometry (Intracellular) - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)
      Flow Cytometry (Intracellular) - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)

      Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling RhoA + RhoC with ab187026 at 1/400 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. 

       

       

    • Immunoprecipitation - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)
      Immunoprecipitation - Anti-RhoA + RhoC antibody [EPR18133] (ab187026)

      Immunoprecipitation of RhoA + RhoC from 1mg of NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate achieved using ab187026 at 1/50 dilution.
      Lane 1: Input: 10µg of NIH/3T3 whole cell lysate.
      Lane 2: NIH/3T3 whole cell lysate following IP with ab187026.
      Lane 3: Negative control: IP using Rabbit monoclonal IgG (ab172730) instead of ab187026 in NIH/3T3 whole cell lysates.
      Western blot was performed using ab187026 at 1/1000 dilution.
      VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1500 was used for detection.
      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 30 second exposure.

    • Anti-RhoA + RhoC antibody [EPR18133] (ab187026)
      Anti-RhoA + RhoC antibody [EPR18133] (ab187026)

    参考文献 (3)

    ab187026 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab187026 は 3 報の論文で使用されています。

    • Tian L  et al. Berberine elevates mitochondrial membrane potential and decreases reactive oxygen species by inhibiting the Rho/ROCK pathway in rats with diabetic encephalopathy. Mol Pain 17:1744806921996101 (2021). PubMed: 33632015
    • Ge Z  et al. Overexpression of the hyperplasia suppressor gene inactivates airway fibroblasts obtained from a rat model of chronic obstructive pulmonary disease by inhibiting the Wnt signaling pathway. Mol Med Rep 20:2754-2762 (2019). PubMed: 31322244
    • Liu AH  et al. The roles of interleukin-1 and RhoA signaling pathway in rat epilepsy model treated with low-frequency electrical stimulation. J Cell Biochem 119:2535-2544 (2018). PubMed: 28941295

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab187026.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com