Anti-RHEB 抗体 (ab25873)
Key features and details
- Rabbit polyclonal to RHEB
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human, Drosophila melanogaster
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-RHEB antibody
RHEB 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RHEB -
由来種
Rabbit -
特異性
ab25873 recognises Rheb (Ras homolog enriched in brain). -
アプリケーション
適用あり: IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human, Drosophila melanogaster -
免疫原
A 15 amino acid peptide from the middle region of Human Rheb.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab25873の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 2 µg/ml.
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| ICC/IF |
Use at an assay dependent concentration.
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| WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa.
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| 特記事項 |
|---|
|
IHC-P
Use a concentration of 2 µg/ml. |
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ICC/IF
Use at an assay dependent concentration. |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa. |
ターゲット情報
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機能
Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity. -
組織特異性
Ubiquitous. Highest levels observed in skeletal and cardiac muscle. -
配列類似性
Belongs to the small GTPase superfamily. Rheb family. -
翻訳後修飾
Farnesylation is important for efficiently activating mTORC1-mediated signaling. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 6009 Human
- Entrez Gene: 19744 Mouse
- Entrez Gene: 26954 Rat
- Omim: 601293 Human
- SwissProt: Q15382 Human
- SwissProt: Q921J2 Mouse
- SwissProt: Q62639 Rat
- Unigene: 283521 Human
see all -
別名
- Ras homolog enriched in brain 2, formerly antibody
- GTP binding protein Rheb antibody
- GTP-binding protein Rheb antibody
see all
画像
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Western blot - Anti-RHEB antibody (ab25873)Western blot analysis of Rheb in mouse brain cell lysate with ab25873 at (A) 1, (B) 2, and (C) 4 ug/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHEB antibody (ab25873)ab25873 at 2µg/ml staining RHEB in mouse brain tissue by IHC
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab25873 は 17 報の論文で使用されています。
- Sun X et al. Mechanistic target of rapamycin complex 1 orchestrates the interplay between hepatocytes and Kupffer cells to determine the outcome of immune-mediated hepatitis. Cell Death Dis 13:1031 (2022). PubMed: 36494334
- Lu Q et al. Complement factor B in high glucose-induced podocyte injury and diabetic kidney disease. JCI Insight 6:N/A (2021). PubMed: 34622800
- Li Z et al. miR-155-5p upregulation ameliorates myocardial insulin resistance via mTOR signaling in chronic alcohol drinking rats. PeerJ 9:e10920 (2021). PubMed: 33868799
- Tang L et al. Electroacupuncture Upregulated Ghrelin in Rats with Functional Dyspepsia via AMPK/TSC2/Rheb-Mediated mTOR Inhibition. Dig Dis Sci 65:1689-1699 (2020). PubMed: 31863340
- Li Z et al. miR-155-5p alleviates ethanol-induced myocardial insulin resistance in H9C2 cells via regulating the mTOR signalling pathway. Mol Biol Rep 47:9469-9477 (2020). PubMed: 33159675