Anti-RHCG 抗体 (ab187904)
Key features and details
- Rabbit polyclonal to RHCG
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-RHCG antibody
RHCG 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RHCG -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human RHCG aa 1-100.
Database link: Q9UBD6 -
ポジティブ･コントロール
- WB: Human plasma, Human tonsil. IHC-P: Human tonsil, Esophagus, kidney tissues.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187904の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 53 kDa.
|
|
| IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 53 kDa. |
|
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Functions as an electroneutral and bidirectional ammonium transporter. May regulate transepithelial ammonia secretion. -
組織特異性
Expressed in brain, testis, placenta, pancreas, esophagus and prostate. Expressed in squamous epithelial tissues (at protein level). According to PubMed:11062476, specifically expressed in kidney. -
配列類似性
Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily. -
発生段階
Specifically expressed in fetal kidney. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Apical cell membrane. Also detected at the basolateral membrane and in subapical vesicles. - Information by UniProt
-
参照データベース
- Entrez Gene: 51458 Human
- Omim: 605381 Human
- SwissProt: Q9UBD6 Human
- Unigene: 459284 Human
-
別名
- Ammonium transporter Rh type C antibody
- C15orf6 antibody
- CDRC2 antibody
see all
画像
-
Western blot - Anti-RHCG antibody (ab187904)All lanes : Anti-RHCG antibody (ab187904) at 1/100 dilution
Lane 1 : Human cell line RT-4 lysate
Lane 2 : Human cell line U-251MG sp lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique.
Predicted band size: 53 kDa
Exposure time: 1 minute -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHCG antibody (ab187904)Immunohistochemical analysis showing RHCG staining in paraffin embedded Human Esophagus tissue using ab187904 at 1/1000 dilution showing positive staining in squamous epithelial cells.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHCG antibody (ab187904)Immunohistochemical analysis showing RHCG staining in paraffin embedded Human tonsil tissue using ab187904 at 1/1000 dilution showing positive staining in squamous epithelial cells.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHCG antibody (ab187904)Immunohistochemical analysis showing RHCG staining in paraffin embedded Human kidney tissue using ab187904 at 1/1000 dilution showing positive staining in distal tubule cells.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHCG antibody (ab187904)Immunohistochemical analysis of paraffin embedded Human lymph node tissue (Negative control) using ab187904 at 1/1000 dilution showing no staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHCG antibody (ab187904)Immunohistochemical analysis of paraffin embedded Human pancrease tissue (Negative control) using ab187904 at 1/1000 dilution showing no staining.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab187904 は 1 報の論文で使用されています。
- Zhang K et al. CircATIC inhibits esophageal carcinoma progression and promotes radiosensitivity by elevating RHCG through sponging miR-10-3p. Thorac Cancer 13:934-946 (2022). PubMed: 35307984