Anti-RHBDD2 抗体 (ab185448)
Key features and details
- Rabbit polyclonal to RHBDD2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RHBDD2 antibody
RHBDD2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RHBDD2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Recombinant fragment corresponding to Human RHBDD2 aa 200-300.
Database link: Q6NTF9 -
ポジティブ･コントロール
- Human cerebral cortex tissue; U-251 MG cells; RHBDD2 overexpression HEK293T cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185448の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 39 kDa.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
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| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 39 kDa. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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配列類似性
Belongs to the peptidase S54 family. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 57414 Human
- Entrez Gene: 215160 Mouse
- Omim: 615203 Human
- SwissProt: Q6NTF9 Human
- SwissProt: Q8VEK2 Mouse
- Unigene: 488827 Human
- Unigene: 295438 Mouse
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別名
- H_RG122E10.2a antibody
- H_RG122E10.2b antibody
- NPD007 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-RHBDD2 antibody (ab185448)
Immunofluorescent analysis of PFA/Triton X-100-treated U-251 MG cells labeling RHBDD2 with ab185448 at 4 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHBDD2 antibody (ab185448)Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling RHBDD2 with ab185448 at 1/200 dilution.
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Western blot - Anti-RHBDD2 antibody (ab185448)All lanes : Anti-RHBDD2 antibody (ab185448) at 1/100 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).
Predicted band size: 39 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHBDD2 antibody (ab185448)Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling RHBDD2 with ab185448 at 1/200 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab185448 は論文での使用が確認できていません。