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リコンビナントRabMAb

Anti-RGS9 抗体 [EPR2873] (ab108975)

Submit a review Submit a question References (1)
Western blot - Anti-RGS9 antibody [EPR2873] (ab108975)
  • Western blot - Anti-RGS9 antibody [EPR2873] (ab108975)
  • Immunocytochemistry/ Immunofluorescence - Anti-RGS9 antibody [EPR2873] (ab108975)
  • Flow Cytometry (Intracellular) - Anti-RGS9 antibody [EPR2873] (ab108975)
  • Western blot - Anti-RGS9 antibody [EPR2873] (ab108975)
  • Flow Cytometry (Intracellular) - Anti-RGS9 antibody [EPR2873] (ab108975)
  • Anti-RGS9 antibody [EPR2873] (ab108975)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2873] to RGS9
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-RGS9 antibody [EPR2873]
    RGS9 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR2873] to RGS9

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, ICC/IFmore details
    適用なし: IHC-P or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Y79 and PC12 cell lysates and human and mouse spleen tissue lysates. ICC/IF: Neuro-2a cells. Flow Cyt (intra): PC12 and Y79 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR2873

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab108975の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/30 - 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 77 kDa).
ICC/IF
1/50 - 1/100.
特記事項
Flow Cyt (Intra)
1/30 - 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 77 kDa).
ICC/IF
1/50 - 1/100.
追加情報
Is unsuitable for IHC-P or IP.

ターゲット情報

  • 機能

    Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(t)-alpha. Involved in phototransduction; key element in the recovery phase of visual transduction.

  • 組織特異性

    Highly expressed in the caudate and putamen, lower levels found in the hypothalamus and nucleus accumbens and very low levels in cerebellum. Not expressed in globus pallidus or cingulate cortex. Isoform 2 is expressed predominantly in pineal gland and retina. Isoform 3 is expressed in retina (abundant in photoreceptors).

  • 関連疾患

    Defects in RGS9 are a cause of prolonged electroretinal response suppression (PERRS) [MIM:608415]; also known as bradyopsia. PERRS is characterized by difficulty adjusting to sudden changes in luminance levels mediated by cones.

  • 配列類似性

    Contains 1 DEP domain.
    Contains 1 G protein gamma domain.
    Contains 1 RGS domain.

  • ドメイン

    In photoreceptor cells the DEP domain is essential for targeting RGS9 to the outer rod segments.

  • 翻訳後修飾

    Retinal isoform 3 is light-dependent phosphorylated at 'Ser-478'. Phosphorylation is decreased by light exposition.

  • 細胞内局在

    Membrane. Isoform 3 is targeted to the membrane via its interaction with RGS9BP.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • PERRS antibody
    • R9AP antibody
    • Regulator of G protein signalling 9 antibody
    • Regulator of G-protein signaling 9 antibody
    • RGS9 antibody
    • RGS9_HUMAN antibody
    • RGS9L antibody
    see all

画像

  • Western blot - Anti-RGS9 antibody [EPR2873] (ab108975)
    Western blot - Anti-RGS9 antibody [EPR2873] (ab108975)
    All lanes : Anti-RGS9 antibody [EPR2873] (ab108975) at 1/1000 dilution (purified)

    Lane 1 : Y79 whole cell lysate
    Lane 2 : Mouse spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 77 kDa
    Observed band size: 55 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.
  • Western blot - Anti-RGS9 antibody [EPR2873] (ab108975)
    Western blot - Anti-RGS9 antibody [EPR2873] (ab108975)
    Anti-RGS9 antibody [EPR2873] (ab108975) at 1/1000 dilution (purified) + PC-12 whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 77 kDa
    Observed band size: 55 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.
  • Immunocytochemistry/ Immunofluorescence - Anti-RGS9 antibody [EPR2873] (ab108975)
    Immunocytochemistry/ Immunofluorescence - Anti-RGS9 antibody [EPR2873] (ab108975)

    Immunocytochemistry/Immunofluorescence analysis of Neuro-2a cells labelling RGS9 with purified ab108975 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

  • Flow Cytometry (Intracellular) - Anti-RGS9 antibody [EPR2873] (ab108975)
    Flow Cytometry (Intracellular) - Anti-RGS9 antibody [EPR2873] (ab108975)

    Intracellular Flow Cytometry analysis of Y79 cells labelling RGS9 with purified ab108975 at a dilution of1/60 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluorr® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies. 

     

     

  • Western blot - Anti-RGS9 antibody [EPR2873] (ab108975)
    Western blot - Anti-RGS9 antibody [EPR2873] (ab108975)
    All lanes : Anti-RGS9 antibody [EPR2873] (ab108975) at 1/1000 dilution (unpurified)

    Lane 1 : PC12 lysates
    Lane 2 : Human spleen lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 77 kDa
    Observed band size: 55 kDa why is the actual band size different from the predicted?



    Note: Predicted molecular weights for UniProt ID O75916 isoforms 1 to 4 are ~77, 55, 57 and 50 kDa respectively. Therefore, the observed WB band may correspond to isoform 2, 3 or 4.
  • Flow Cytometry (Intracellular) - Anti-RGS9 antibody [EPR2873] (ab108975)
    Flow Cytometry (Intracellular) - Anti-RGS9 antibody [EPR2873] (ab108975)

    Intracellular flow cytometric analysis ofpermeabilized PC12 cells labelling RGS9with unpurified ab108975 (red) at a dilution of 1/30 or a rabbit IgG (negative) (green). 

     

     

  • Anti-RGS9 antibody [EPR2873] (ab108975)
    Anti-RGS9 antibody [EPR2873] (ab108975)

参考文献 (1)

ab108975 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108975 は 1 報の論文で使用されています。

  • Vogt A  et al. Isotope coded protein labeling coupled immunoprecipitation (ICPL-IP): a novel approach for quantitative protein complex analysis from native tissue. Mol Cell Proteomics 12:1395-406 (2013). PubMed: 23268931

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