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リコンビナントRabMAb

Anti-RGS10 抗体 [EPR10657(B)] (ab154172)

Submit a review Submit a question References (1)
Western blot - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
  • Immunocytochemistry/ Immunofluorescence - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
  • Anti-RGS10 antibody [EPR10657(B)] (ab154172)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10657(B)] to RGS10
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-RGS10 antibody [EPR10657(B)]
    RGS10 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR10657(B)] to RGS10

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Jurkat and K562 cell lysates; Human prostate tissue; K562 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20ºC.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR10657(B)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab154172の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/50 - 1/100.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/50 - 1/100.
追加情報
Is unsuitable for Flow Cyt.

ターゲット情報

  • 機能

    Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Associates specifically with the activated forms of the G protein subunits G(i)-alpha and G(z)-alpha but fails to interact with the structurally and functionally distinct G(s)-alpha subunit. Activity on G(z)-alpha is inhibited by palmitoylation of the G-protein.

  • 配列類似性

    Contains 1 RGS domain.

  • 翻訳後修飾

    Isoform 3 is phosphorylated on Ser-16.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Regulator of G protein signaling 10 antibody
      • Regulator of G-protein signaling 10 antibody
      • RGS10 antibody
      • RGS10_HUMAN antibody

    画像

    • Western blot - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
      Western blot - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
      All lanes : Anti-RGS10 antibody [EPR10657(B)] (ab154172) at 1/1000 dilution

      Lane 1 : Jurkat cell lysates
      Lane 2 : K562 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 20 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)

      Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling RGS10 with ab154172 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
      Immunocytochemistry/ Immunofluorescence - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
      Immunofluorescent analysis of K562 cells labeling RGS10 with ab154172 at 1/50 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)

      Immunohistochemical analysis of paraffin embedded Human normal tonsil tissue using ab154172 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)

      Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab154172 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)

      Immunohistochemical analysis of paraffin embedded Human gastric adenocarcinoma tissue using ab154172 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-RGS10 antibody [EPR10657(B)] (ab154172)
      Anti-RGS10 antibody [EPR10657(B)] (ab154172)

    参考文献 (1)

    ab154172 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab154172 は 1 報の論文で使用されています。

    • Varland S  et al. N-terminal acetylation shields proteins from degradation and promotes age-dependent motility and longevity. Nat Commun 14:6774 (2023). PubMed: 37891180

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