Anti-RGS1 抗体 (ab117077)
Key features and details
- Goat polyclonal to RGS1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RGS1 antibody
RGS1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to RGS1 -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Horse, Cow, Dog
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免疫原
Synthetic peptide:
C-NLLNDLQANSLK
(with a Cysteine residue linker), corresponding to C terminal amino acids 198-209 of Human RGS1 (Q08116). -
ポジティブ･コントロール
- Recombinant Human RGS1 protein (ab126697) can be used as a positive control in WB. Hep G2 lysate, Human Small Intestine and Human Thymus tissues
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab117077の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 24 kDa.
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| IHC-P |
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 24 kDa. |
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IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. This protein may be involved in the regulation of B-cell activation and proliferation. -
組織特異性
B-cell specific. Expression is relatively low in B-cells and chronic lymphocytic leukemia B-cells; however, in other types of malignant B-cell such as non-Hodgkin lymphoma and hairy cell leukemia, expression is constitutively high. -
配列類似性
Contains 1 RGS domain. -
翻訳後修飾
Could be phosphorylated. Might be functionally regulated by protein kinase(s). - Information by UniProt
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参照データベース
- Entrez Gene: 100034151 Horse
- Entrez Gene: 5996 Human
- Entrez Gene: 50778 Mouse
- Entrez Gene: 54289 Rat
- Omim: 600323 Human
- SwissProt: Q6RG78 Horse
- SwissProt: Q08116 Human
- SwissProt: Q9JL25 Mouse
see all -
別名
- 1R20 antibody
- B cell activation protein BL34 antibody
- B-cell activation protein BL34 antibody
see all
画像
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Western blot - Anti-RGS1 antibody (ab117077)Anti-RGS1 antibody (ab117077) at 1 µg/ml + Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 24 kDaPrimary incubation 1 hour at room temperature. Detected by chemiluminescence. RIPA buffer used.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS1 antibody (ab117077)Human Small Intestine (formalin-fixed, paraffin-embedded) stained with ab117077 followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS1 antibody (ab117077)Human Thymus (formalin-fixed, paraffin-embedded) stained with ab117077 followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab117077 は 2 報の論文で使用されています。
- Li J et al. Biliverdin Protects Against Cerebral Ischemia/Reperfusion Injury by Regulating the miR-27a-3p/Rgs1 Axis. Neuropsychiatr Dis Treat 17:1165-1181 (2021). PubMed: 33911865
- Wang Y et al. Long non-coding RNA TUG1 recruits miR-29c-3p from its target gene RGS1 to promote proliferation and metastasis of melanoma cells. Int J Oncol 54:1317-1326 (2019). PubMed: 30720136