Anti-RFX5 抗体 (ab9255)
Key features and details
- Rabbit polyclonal to RFX5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-RFX5 antibody
RFX5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RFX5 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Gorilla
-
免疫原
Synthetic peptide (Human) conjugated to KLH - which represents aportion of human Regulatory Factor X 5 (GenBank PID 4557543).
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Antibodies were affinity purified using thepeptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nmof 1.4 equals 1.0 mg of IgG. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9255の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/5000. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
|
| 特記事項 |
|---|
|
WB
1/500 - 1/5000. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa). |
ターゲット情報
-
機能
Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters. -
組織特異性
Ubiquitous. -
関連疾患
Defects in RFX5 are a cause of bare lymphocyte syndrome type 2 (BLS2) [MIM:209920]; also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections. -
配列類似性
Belongs to the RFX family.
Contains 1 RFX-type winged-helix DNA-binding domain. -
ドメイン
The N-terminus is required for dimer formation, association with RFXANK and RFXAP, assembly of the RFX complex, and for binding of this complex to its X box target site in the MHC-II promoter. The C-terminus mediates cooperative binding between the RFX complex and NF-Y. -
翻訳後修飾
Phosphorylated. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 5993 Human
- Omim: 601863 Human
- SwissProt: P48382 Human
- Unigene: 632472 Human
-
別名
- DNA binding protein RFX 5 antibody
- DNA binding protein RFX5 antibody
- DNA-binding protein RFX5 antibody
see all
画像
-
Western blot - Anti-RFX5 antibody (ab9255)ab9255 at a 1:1000 dilution staining RFX5 in 500
µ g of HeLa Nuclear Extract by Western Blot (ECL). Immunoprecipitation was performed prior to Western Blotting with 2.0µ g of ab9255.
ab9255 at a 1:1000 dilution staining RFX5 in 500µg of HeLa Nuclear Extract by Western Blot (ECL). Immunoprecipitation was performed prior to Western Blotting with 2.0µg of ab9255.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab9255 は 4 報の論文で使用されています。
- Shen H et al. ZEB1-induced LINC01559 expedites cell proliferation, migration and EMT process in gastric cancer through recruiting IGF2BP2 to stabilize ZEB1 expression. Cell Death Dis 12:349 (2021). PubMed: 33824282
- Mueller PA et al. Deletion of Macrophage Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Accelerates Atherosclerosis Regression and Increases C-C Chemokine Receptor Type 7 (CCR7) Expression in Plaque Macrophages. Circulation 138:1850-1863 (2018). PubMed: 29794082
- Chen L et al. Juglanin inhibits lung cancer by regulation of apoptosis, ROS and autophagy induction. Oncotarget 8:93878-93898 (2017). PubMed: 29212196
- Wolf K et al. Treatment of Chlamydia trachomatis with a small molecule inhibitor of the Yersinia type III secretion system disrupts progression of the chlamydial developmental cycle. Mol Microbiol 61:1543-55 (2006). PubMed: 16968227