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リコンビナントRabMAb

Anti-RFP 抗体 [EPR18992] (ab185921)

Submit a review Submit a question References (6)
Western blot - Anti-RFP antibody [EPR18992] (ab185921)
  • Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody [EPR18992] (ab185921)
  • Western blot - Anti-RFP antibody [EPR18992] (ab185921)
  • Anti-RFP antibody [EPR18992] (ab185921)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18992] to RFP
  • Suitable for: ICC/IF, WB
  • Reacts with: Species independent

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-RFP antibody [EPR18992]
    RFP 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR18992] to RFP

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, WBmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Recombinant full length protein within RFP aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (Entacmaea quadricolor)
    Database link: Q8ISF8

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: RFP transfected HEK-293 whole cell lysate. ICC/IF: RFP transfected HEK-293 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR18992

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab185921の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/100.
WB
1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
特記事項
ICC/IF
1/100.
WB
1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).

ターゲット情報

  • 関連性

    Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

  • 別名

    • DsRed antibody
    • GFP like chromoprotein antibody
    • red fluorescent protein antibody
    • RFP antibody

画像

  • Western blot - Anti-RFP antibody [EPR18992] (ab185921)
    Western blot - Anti-RFP antibody [EPR18992] (ab185921)
    All lanes : Anti-RFP antibody [EPR18992] (ab185921) at 1/1000 dilution

    Lane 1 : HEK-293T mock transfected cell lysate
    Lane 2 : HEK293 RFP transfected cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 26 kDa
    Observed band size: 30 kDa why is the actual band size different from the predicted?



    Western blot: Anti-RFP antibody [EPR18992] (ab185921) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab185921 was shown to bind specifically to RFP. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody [EPR18992] (ab185921)
    Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody [EPR18992] (ab185921)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Empty vector transfected or RFP transfected HEK-293 (Human epithelial cell line from embryonic kidney) cells labeling RFP with ab185921 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing positive staining after transfection of RFP on HEK-293 cells. 

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [EPR18992] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab185921 at 1/100 dilution followed by ab150120 at 1/1000 dilution.

    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077  at 1/1000 dilution.

  • Western blot - Anti-RFP antibody [EPR18992] (ab185921)
    Western blot - Anti-RFP antibody [EPR18992] (ab185921)
    All lanes : Anti-RFP antibody [EPR18992] (ab185921) at 1/10000 dilution

    Lane 1 : RFP transfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 2 : Empty vector (vector control) transfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 5 : Human kidney lysate
    Lane 6 : Human liver lysate
    Lane 7 : Mouse brain lysate
    Lane 8 : Mouse heart lysate
    Lane 9 : Rat brain lysate
    Lane 10 : Rat heart lysate
    Lane 11 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 12 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 13 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 14 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 26 kDa
    Observed band size: 26 kDa


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-RFP antibody [EPR18992] (ab185921)
    Anti-RFP antibody [EPR18992] (ab185921)

参考文献 (6)

ab185921 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab185921 は 6 報の論文で使用されています。

  • Kong W  et al. A high-efficient and naked-eye visible CRISPR/Cas9 system in Arabidopsis. Planta 257:30 (2023). PubMed: 36596996
  • Cheng N  et al. Senescent hepatic stellate cells promote liver regeneration through IL-6 and ligands of CXCR2. JCI Insight 7:N/A (2022). PubMed: 35708907
  • Zheng W  et al. African Swine Fever Virus Structural Protein p17 Inhibits cGAS-STING Signaling Pathway Through Interacting With STING. Front Immunol 13:941579 (2022). PubMed: 35844609
  • Kolpakova J  et al. Binge alcohol drinking alters the differential control of cholinergic interneurons over nucleus accumbens D1 and D2 medium spiny neurons. Front Cell Neurosci 16:1010121 (2022). PubMed: 36589290
  • Sui S  et al. iPSC-Derived Trabecular Meshwork Cells Stimulate Endogenous TM Cell Division Through Gap Junction in a Mouse Model of Glaucoma. Invest Ophthalmol Vis Sci 62:28 (2021). PubMed: 34427623
  • Xia N  et al. African Swine Fever Virus Structural Protein p17 Inhibits Cell Proliferation through ER Stress-ROS Mediated Cell Cycle Arrest. Viruses 13:N/A (2020). PubMed: 33374251

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