Anti-RFP 抗体 (ab28664)
Key features and details
- Rabbit polyclonal to RFP
- Suitable for: WB, ICC/IF
- Reacts with: Species independent
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-RFP antibody
RFP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RFP -
由来種
Rabbit -
特異性
ab28664 detects RFP tagged proteins. This antibody is specific for the RFP tag. It shows very low reactivity to GFP or GFP tagged proteins. ab28664 non-specifically detects a 45 kDa protein from E. coli extracts. This non-specific cross-reactivity is not present in mammalian samples. -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide:
VNGHEFEIEGEGEGR
, corresponding to amino acids 22-36 of RFP from Discosoma sea anemone. -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab28664の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
1/1000. Predicted molecular weight: 26 kDa.
Use at a concentration of 2 µg/ml. Block membrane in 5% milk and incubate primary and secondary antibodies in milk. |
ICC/IF | (1) |
1/50 - 1/200.
|
特記事項 |
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WB
1/1000. Predicted molecular weight: 26 kDa. Use at a concentration of 2 µg/ml. Block membrane in 5% milk and incubate primary and secondary antibodies in milk. |
ICC/IF
1/50 - 1/200. |
ターゲット情報
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関連性
Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. -
別名
- GFP like chromoprotein antibody
- red fluorescent protein antibody
- RFP antibody
画像
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Western blot of recombinant RFP using ab28664.
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Immunofluorescent analysis of recombinant Red Fluorescent Protein (RFP)-transfected HeLa cells. Cells were transfected with a pCMV RFP C-Myc plasmid and 48 hours post-transfection cells were fixed with formalin and permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were blocked with 3% BSA for 15 minutes at room temperature, and then probed with ab28664 at a dilution of 1/50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight® 488-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1/400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (14)
ab28664 は 14 報の論文で使用されています。
- Cha M et al. Quantitative imaging of vesicle-protein interactions reveals close cooperation among proteins. J Extracell Vesicles 12:e12322 (2023). PubMed: 37186457
- Hirschenberger M et al. ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling. Nat Commun 14:6770 (2023). PubMed: 37914730
- Schwirz J et al. Bicistronic expression and differential localization of proteins in insect cells and Drosophila suzukii using picornaviral 2A peptides. Insect Biochem Mol Biol 119:103324 (2020). PubMed: 31978587
- Chang X et al. PADI3 induces cell cycle arrest via the Sirt2/AKT/p21 pathway and acts as a tumor suppressor gene in colon cancer. Cancer Biol Med 16:729-742 (2019). PubMed: 31908891
- Chai Z et al. PADI3 plays an antitumor role via the Hsp90/CKS1 pathway in colon cancer. Cancer Cell Int 19:277 (2019). PubMed: 31708688