Anti-RFP 抗体 (ab152123)
Key features and details
- Rabbit polyclonal to RFP
- Suitable for: WB, IP, ICC/IF
- Reacts with: Species independent
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-RFP antibody
RFP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RFP -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, ICC/IFmore details -
種交差性
交差種: Species independent -
免疫原
Recombinant full length RFP.
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ポジティブ･コントロール
- RFP-transfected HeLa cells; RFP-transfected 293T lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab152123の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/5000 - 1/20000. Predicted molecular weight: 27 kDa.
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| IP |
1/10000.
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| ICC/IF |
1/5000.
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| 特記事項 |
|---|
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WB
1/5000 - 1/20000. Predicted molecular weight: 27 kDa. |
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IP
1/10000. |
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ICC/IF
1/5000. |
ターゲット情報
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関連性
Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. -
別名
- GFP like chromoprotein antibody
- red fluorescent protein antibody
- RFP antibody
画像
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Western blot - Anti-RFP antibody (ab152123)All lanes : Anti-RFP antibody (ab152123) at 1/10000 dilution
Lane 1 : Non-transfected 293T whole cell lysate
Lane 2 : GFP-transfected 293T whole cell lysate
Lane 3 : RFP-transfected 293T whole cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 27 kDa
12% SDS PAGE -
Immunoprecipitation - Anti-RFP antibody (ab152123)Immunoprecipitation of RFP-tagged protein.
HeLa transfected lysates were subjected to immunoprecipitation using:
Lane 1: 15 µg of HeLa transfected lysate
Lane 2: normal rabbit IgG
Lane 3: 2.5 µg ab152123
Detection ab152123 at 1/10000 dilution -
Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody (ab152123)Immunofluorescence analysis of RFP-transfected HeLa cells labeling RFP with ab152123: (1) RFP is expressed in the tranfected cell, (2) The cell expressing RFP is detected using ab152123 at 1/5000 followed by Alexa Fluor 488 at 1/500 Goat anti-Rabbit IgG, (3) Merged with DNA probe, the lower cell represents a negtive control.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab152123 は 3 報の論文で使用されています。
- Zhou Y et al. Orf Virus ORF120 Protein Positively Regulates the NF-κB Pathway by Interacting with G3BP1. J Virol 95:e0015321 (2021). PubMed: 34287041
- Lu X et al. Lentivirus-mediated IL-10-expressing Bone Marrow Mesenchymal Stem Cells promote corneal allograft survival via upregulating lncRNA 003946 in a rat model of corneal allograft rejection. Theranostics 10:8446-8467 (2020). PubMed: 32724480
- Joseph SR et al. Competition between histone and transcription factor binding regulates the onset of transcription in zebrafish embryos. Elife 6:N/A (2017). IP . PubMed: 28425915